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ZFIN ID: ZDB-FIG-180426-15
Bisgrove et al., 2017 - Maternal Gdf3 is an obligatory cofactor in nodal signaling for embryonic axis formation in zebrafish. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 90%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage: 90%-epiboly

Fig. 2

Gdf3 is required for mesoderm, endoderm and neural patterning.

(A–J’) WISH analysis of gene expression in embryos at 90% epiboly. Columns from left to right show WT embryos, WT embryos injected with 100 pg of gdf3 RNA, MZgdf3 mutants and MZgdf3 injected with gdf3 RNA. Each panel is a representative image of at least 15 embryos examined. (A–L) Midline and margin expression of Nodal signaling pathway genes ndr2 (A–D) and Lefty family members lft1 (E–H) and lft2 (I–L) were absent in MZgdf3 mutants and restored by gdf3 mRNA injection. (M–X) Analysis of early mesoderm transcription factor genes. (M–T). Expression domains of gsc, ta (ntl) and tbx16 (spt) were absent from the midline of MZgdf3 mutants, but restored by gdf3 RNA injection. (Q–X) Lateral and ventral mesendoderm expression domains of tbx16 (Q–T), and eve1 (U–X), which were reduced in width in MZgdf3, were restored to wild-type levels by gdf3 RNA. (Y–F’) Endoderm expression domains of transcription factors sox17 (Y–B’) and foxa2 (C’–F’) were absent in MZgdf3, and restored by gdf3 RNA, as was expression of foxa2 in midline neural tissues. (G’–J’). otx2 expression in the anterior neural plate is reduced in MZgdf3 but rescued to its normal extent by gdf3 RNA. (Second column from left) Strikingly, although injection of gdf3 RNA was capable of rescuing mesoderm, endoderm and neural tissues in MZgdf3 mutants it had no effect on gene expression in WT embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
eve1 AB standard conditions 90%-epiboly margin ventral region ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly margin ventral region ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly margin ventral region ISH
foxa2 AB standard conditions 90%-epiboly axial hypoblast ISH
90%-epiboly endoderm ISH
90%-epiboly neuroectoderm ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly Not Detected axial hypoblast ISH
90%-epiboly Not Detected endoderm ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly Not Detected axial hypoblast ISH
90%-epiboly Not Detected endoderm ISH
gsc AB standard conditions 90%-epiboly neuroectoderm ISH
90%-epiboly prechordal plate ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly Not Detected neuroectoderm ISH
90%-epiboly Not Detected prechordal plate ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly Not Detected neuroectoderm ISH
90%-epiboly Not Detected prechordal plate ISH
lft1 AB standard conditions 90%-epiboly axial chorda mesoderm ISH
90%-epiboly hypoblast ISH
90%-epiboly neuroectoderm ISH
90%-epiboly prechordal plate ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly Not Detected axial chorda mesoderm ISH
90%-epiboly Not Detected hypoblast ISH
90%-epiboly Not Detected neuroectoderm ISH
90%-epiboly Not Detected prechordal plate ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly Not Detected axial chorda mesoderm ISH
90%-epiboly Not Detected hypoblast ISH
90%-epiboly Not Detected neuroectoderm ISH
90%-epiboly Not Detected prechordal plate ISH
lft2 AB standard conditions 90%-epiboly neuroectoderm ISH
90%-epiboly prechordal plate ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly Not Detected neuroectoderm ISH
90%-epiboly Not Detected prechordal plate ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly Not Detected neuroectoderm ISH
90%-epiboly Not Detected prechordal plate ISH
ndr2 AB standard conditions 90%-epiboly axial mesoderm anterior region ISH
90%-epiboly axial mesoderm posterior region ISH
90%-epiboly neuroectoderm ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly Not Detected axial mesoderm anterior region ISH
90%-epiboly Not Detected axial mesoderm posterior region ISH
90%-epiboly Not Detected neuroectoderm ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly Not Detected axial mesoderm anterior region ISH
90%-epiboly Not Detected axial mesoderm posterior region ISH
90%-epiboly Not Detected neuroectoderm ISH
otx2b AB standard conditions 90%-epiboly anterior neural plate ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly anterior neural plate ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly anterior neural plate ISH
sox17 AB standard conditions 90%-epiboly endoderm ISH
90%-epiboly forerunner cell group ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly endoderm ISH
90%-epiboly forerunner cell group ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly endoderm ISH
90%-epiboly forerunner cell group ISH
tbx16 AB standard conditions 90%-epiboly hypoblast ISH
90%-epiboly prechordal plate ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly Not Detected hypoblast ISH
90%-epiboly prechordal plate ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly Not Detected hypoblast ISH
90%-epiboly prechordal plate ISH
tbxta AB standard conditions 90%-epiboly axial chorda mesoderm ISH
gdf3zy51/zy51(AB) standard conditions 90%-epiboly Not Detected axial chorda mesoderm ISH
gdf3zy52/zy52(AB) standard conditions 90%-epiboly Not Detected axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gdf3zy51/zy51(AB) standard conditions 90%-epiboly anterior neural plate otx2b expression decreased distribution, abnormal
90%-epiboly axial chorda mesoderm tbxta expression absent, abnormal
90%-epiboly axial chorda mesoderm lft1 expression absent, abnormal
90%-epiboly axial hypoblast foxa2 expression absent, abnormal
90%-epiboly axial mesoderm anterior region ndr2 expression absent, abnormal
90%-epiboly axial mesoderm posterior region ndr2 expression absent, abnormal
90%-epiboly endoderm foxa2 expression absent, abnormal
90%-epiboly endoderm sox17 expression decreased amount, abnormal
90%-epiboly forerunner cell group sox17 expression decreased amount, abnormal
90%-epiboly hypoblast lft1 expression absent, abnormal
90%-epiboly hypoblast tbx16 expression absent, abnormal
90%-epiboly margin ventral region eve1 expression decreased distribution, abnormal
90%-epiboly neuroectoderm lft2 expression absent, abnormal
90%-epiboly neuroectoderm ndr2 expression absent, abnormal
90%-epiboly neuroectoderm lft1 expression absent, abnormal
90%-epiboly neuroectoderm gsc expression absent, abnormal
90%-epiboly prechordal plate lft2 expression absent, abnormal
90%-epiboly prechordal plate lft1 expression absent, abnormal
90%-epiboly prechordal plate gsc expression absent, abnormal
90%-epiboly prechordal plate tbx16 expression decreased distribution, abnormal
gdf3zy52/zy52(AB) standard conditions 90%-epiboly anterior neural plate otx2b expression decreased distribution, abnormal
90%-epiboly axial chorda mesoderm tbxta expression absent, abnormal
90%-epiboly axial chorda mesoderm lft1 expression absent, abnormal
90%-epiboly axial hypoblast foxa2 expression absent, abnormal
90%-epiboly axial mesoderm anterior region ndr2 expression absent, abnormal
90%-epiboly axial mesoderm posterior region ndr2 expression absent, abnormal
90%-epiboly endoderm foxa2 expression absent, abnormal
90%-epiboly endoderm sox17 expression decreased amount, abnormal
90%-epiboly forerunner cell group sox17 expression decreased amount, abnormal
90%-epiboly hypoblast lft1 expression absent, abnormal
90%-epiboly hypoblast tbx16 expression absent, abnormal
90%-epiboly margin ventral region eve1 expression decreased distribution, abnormal
90%-epiboly neuroectoderm gsc expression absent, abnormal
90%-epiboly neuroectoderm lft2 expression absent, abnormal
90%-epiboly neuroectoderm ndr2 expression absent, abnormal
90%-epiboly neuroectoderm lft1 expression absent, abnormal
90%-epiboly prechordal plate lft2 expression absent, abnormal
90%-epiboly prechordal plate gsc expression absent, abnormal
90%-epiboly prechordal plate lft1 expression absent, abnormal
90%-epiboly prechordal plate tbx16 expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife