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ZFIN ID: ZDB-FIG-180426-16
Bisgrove et al., 2017 - Maternal Gdf3 is an obligatory cofactor in nodal signaling for embryonic axis formation in zebrafish. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to Prim-5

Fig. 3

Gdf3 is required for mesoderm, endoderm and neural patterning.

(A–J’) WISH analysis of gene expression in embryos at 90% epiboly. Columns from left to right show WT embryos, WT embryos injected with 100 pg of gdf3 RNA, MZgdf3 mutants and MZgdf3 injected with gdf3 RNA. Each panel is a representative image of at least 15 embryos examined. (A–L) Midline and margin expression of Nodal signaling pathway genes ndr2 (A–D) and Lefty family members lft1 (E–H) and lft2 (I–L) were absent in MZgdf3 mutants and restored by gdf3 mRNA injection. (M–X) Analysis of early mesoderm transcription factor genes. (M–T). Expression domains of gsc, ta (ntl) and tbx16 (spt) were absent from the midline of MZgdf3 mutants, but restored by gdf3 RNA injection. (Q–X) Lateral and ventral mesendoderm expression domains of tbx16 (Q–T), and eve1 (U–X), which were reduced in width in MZgdf3, were restored to wild-type levels by gdf3 RNA. (Y–F’) Endoderm expression domains of transcription factors sox17 (Y–B’) and foxa2 (C’–F’) were absent in MZgdf3, and restored by gdf3 RNA, as was expression of foxa2 in midline neural tissues. (G’–J’). otx2 expression in the anterior neural plate is reduced in MZgdf3 but rescued to its normal extent by gdf3 RNA. (Second column from left) Strikingly, although injection of gdf3 RNA was capable of rescuing mesoderm, endoderm and neural tissues in MZgdf3 mutants it had no effect on gene expression in WT embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gsc AB standard conditions Shield germ ring ISH
gdf3zy51/zy51(AB) standard conditions Shield Not Detected germ ring ISH
gdf3zy52/zy52(AB) standard conditions Shield Not Detected germ ring ISH
tbxta AB standard conditions Shield germ ring ISH
gdf3zy51/zy51(AB) standard conditions Shield germ ring ISH
Shield germ ring dorsal region ISH
gdf3zy52/zy52(AB) standard conditions Shield germ ring ISH
Shield germ ring dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gdf3zy51/zy51(AB) standard conditions Shield germ ring gsc expression absent, abnormal
Shield germ ring tbxta expression spatial pattern, abnormal
Shield germ ring dorsal region tbxta expression decreased amount, abnormal
Prim-5 central nervous system anterior region malformed, abnormal
Prim-5 notochord absent, abnormal
Prim-5 whole organism morphology, abnormal
gdf3zy52/zy52(AB) standard conditions Shield germ ring gsc expression absent, abnormal
Shield germ ring tbxta expression spatial pattern, abnormal
Shield germ ring dorsal region tbxta expression decreased amount, abnormal
Prim-5 central nervous system anterior region malformed, abnormal
Prim-5 notochord absent, abnormal
Prim-5 whole organism morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife