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ZFIN ID: ZDB-FIG-180308-7
Zhang et al., 2017 - Endoderm Jagged induces liver and pancreas duct lineage in zebrafish. Nature communications   8:769 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 2

Jag1b and Jag2b are required for intrahepatopancreatic duct lineage specification. ah Whole organ immunofluorescence expression analysis of the 72 hpf foregut endoderm of wild type and jag1b −/− ;jag2b −/− mutant embryos. (a, b Alcam channel removed in bottom panels). Intrahepatic duct cells marked by high Alcama levels (a, green), and by Prox1 (red) but not Hnf4a expression (blue, a), are found in wild type liver (arrowheads, a). These duct cells are not found in the jag1b −/− ;jag2b −/− mutant liver (b, representative sample, n = 13), which is comprised entirely of hepatocytes expressing both Prox1 and Hnf4a. cf Intrapancreatic ductal cells expressing Nkx6.1 (red, c, d) or Pdx1 (red, arrow, e, f) are present in wild type but not in jag1b −/− ;jag2b −/− mutants. Pdx1 expression in Islet1+ cells (blue, arrowheads) and the duodenum (asterisk) is comparable to wild-type siblings, as is expression of ptf1a:GFP+ (green) acinar cells cf and Anxa4+ (red) extrahepatopancreatic duct cells (red, g, h). g, h Anxa4+ (red) extrahepatopancreatic ducts can be found joining the ptf1a:GFP+ (green) pancreatic acinar cells to the fabp10a:DsRed+ hepatocytes (blue). Representative samples from three different jag1b −/+ ;jag2b −/+ in-cross clutches. Scale bars, 50 μM

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
alcama zn-8 WT standard conditions Protruding-mouth intrahepatic bile duct IHC
zn-8 Protruding-mouth Not Detected liver hepatocyte IHC
zn-8 jag2bhu3425/hu3425 ; jag1bb1105/b1105 standard conditions Protruding-mouth Not Detected liver IHC
anxa4 Ab-2F11 gz15Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 ; jh1Tg standard conditions Protruding-mouth extrahepatic duct IHC
Ab-2F11 Protruding-mouth extrapancreatic duct IHC
Ab-2F11 gz15Tg; jh1Tg standard conditions Protruding-mouth extrahepatic duct IHC
Ab-2F11 Protruding-mouth extrapancreatic duct IHC
Ab-2F11 Protruding-mouth intrahepatic bile duct IHC
Ab-2F11 Protruding-mouth intrapancreatic duct IHC
DsRed gz15Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 ; jh1Tg standard conditions Protruding-mouth liver hepatocyte IHC
gz15Tg; jh1Tg standard conditions Protruding-mouth liver hepatocyte IHC
EGFP gz15Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 ; jh1Tg standard conditions Protruding-mouth Not Detected intrahepatic bile duct IHC
Protruding-mouth Not Detected intrapancreatic duct IHC
Protruding-mouth pancreatic acinar cell IHC
gz15Tg; jh1Tg standard conditions Protruding-mouth Not Detected intrahepatic bile duct IHC
Protruding-mouth Not Detected intrapancreatic duct IHC
Protruding-mouth pancreatic acinar cell IHC
jh1Tg standard conditions Protruding-mouth Not Detected intrahepatic bile duct IHC
Protruding-mouth Not Detected intrapancreatic duct IHC
Protruding-mouth pancreatic acinar cell IHC
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth Not Detected intrahepatic bile duct IHC
Protruding-mouth Not Detected intrapancreatic duct IHC
Protruding-mouth pancreatic acinar cell IHC
hnf4a ab1-hnf4a WT standard conditions Protruding-mouth Not Detected intrahepatic bile duct IHC
ab1-hnf4a Protruding-mouth liver hepatocyte IHC
ab1-hnf4a jag2bhu3425/hu3425 ; jag1bb1105/b1105 standard conditions Protruding-mouth liver hepatocyte IHC
nkx6.1 Ab2-nkx6 jh1Tg standard conditions Protruding-mouth intrapancreatic duct IHC
Ab2-nkx6 Protruding-mouth Not Detected pancreatic acinar cell IHC
Ab2-nkx6 jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth Not Detected exocrine pancreas IHC
pdx1 jh1Tg standard conditions Protruding-mouth endocrine pancreas IHC
Protruding-mouth intrapancreatic duct IHC
Protruding-mouth Not Detected pancreatic acinar cell IHC
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth endocrine pancreas IHC
Protruding-mouth Not Detected exocrine pancreas IHC
prox1a Ab3-prox1 WT standard conditions Protruding-mouth intrahepatic bile duct IHC
Ab3-prox1 Protruding-mouth liver hepatocyte IHC
Ab3-prox1 jag2bhu3425/hu3425 ; jag1bb1105/b1105 standard conditions Protruding-mouth liver hepatocyte IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-pan-Cadherin IHC jh1Tg standard conditions Protruding-mouth intrapancreatic duct
IHC jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth Not Detected exocrine pancreas
Ab2-isl IHC jh1Tg standard conditions Protruding-mouth endocrine pancreas
IHC jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth endocrine pancreas
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 ; jh1Tg standard conditions Protruding-mouth exocrine pancreas lacks all parts of type intrapancreatic duct, abnormal
Protruding-mouth exocrine pancreas development decreased process quality, abnormal
jag2bhu3425/hu3425 ; jag1bb1105/b1105 standard conditions Protruding-mouth common bile duct development process quality, normal
Protruding-mouth intrahepatic bile duct development decreased process quality, abnormal
Protruding-mouth liver alcama expression absent, abnormal
Protruding-mouth liver lacks all parts of type intrahepatic bile duct, abnormal
Protruding-mouth liver apoptotic process normal occurrence, normal
Protruding-mouth pancreas apoptotic process normal occurrence, normal
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth exocrine pancreas ab1-pan-cadherin labeling absent, abnormal
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth exocrine pancreas nkx6.1 expression absent, abnormal
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth exocrine pancreas pdx1 expression absent, abnormal
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth exocrine pancreas lacks all parts of type intrapancreatic duct, abnormal
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Protruding-mouth exocrine pancreas development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.