FIGURE

Fig. 8

ID

ZDB-FIG-171207-34

Publication

Lu et al., 2015 - Direct regulation of p53 by miR-142a-3p mediates the survival of hematopoietic stem and progenitor cells in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Combined knockdown of irf7 and p53 restores decreased HSPCs in 142^{T −/−} embryos. (a and b) Injection of irf7 MO with p53 MO rescued the decreased expression of HSPCs marker runx1, and the extent of the restoration was greater than the single injection of irf7 MO at 26 and 36 hpf (mean±s.d., n=5, *P<0.05). (c) The increased expression of p53 was not affected in 142^T−/− embryos injected with irf7 MO at 26 and 36 hpf by qPCR (each sample was composed at least 40 embryos, mean±s.d., n=3, *P<0.05, **P<0.01, ns stands for no significance). (d) The increased expression of irf7 was not affected in 142^T−/− embryos injected with p53 MO at 26 hpf, 2 and 4 dpf by qPCR (mean±s.d., n=3, *P<0.05, **P<0.01, ns stands for no significance).

Expression Data

Genes:	irf7 runx1 tp53
Fish:	WT mir142a^ioz13/ioz13 mir142a^ioz13/ioz13 + MO1-irf7 mir142a^ioz13/ioz13 + MO1-irf7 + MO4-tp53 mir142a^ioz13/ioz13 + MO4-tp53
Knockdown Reagents:	MO1-irf7 MO4-tp53
Anatomical Terms:	trunk ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mir142a^ioz13/ioz13 mir142a^ioz13/ioz13 + MO1-irf7 mir142a^ioz13/ioz13 + MO1-irf7 + MO4-tp53 mir142a^ioz13/ioz13 + MO4-tp53
Knockdown Reagents:	MO1-irf7 MO4-tp53
Observed In:	trunk ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Discov