FIGURE

Fig. 7

ID

ZDB-FIG-171207-33

Publication

Lu et al., 2015 - Direct regulation of p53 by miR-142a-3p mediates the survival of hematopoietic stem and progenitor cells in zebrafish

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Fig. 7

P53 mediates the decrease of HSPCs in miR-142a-3p mutants. (a) P53 MO-injected 142^T−/− embryos showed the restoration of runx1 expression at 26 and 36 hpf by WISH (left panel). The quantification of runx1-positive cells in the AGM was shown in the right panel (mean±s.d., n=3, *P<0.05). (b) qPCR showed that compared with 142^T−/− at 36 hpf, the decrease in runx1 expression was rescued in 142^{T −/−} embryos injected with p53 MO (mean±s.d., n=3, *P<0.05). (c) WISH showed the expression of runx1 in 142^T+/+ p53^+/+, 142^T−/− p53^+/+ and 142^T−/− p53^−/− embryos at 36 hpf. There was a rescue effect on the decreased runx1 expression in 142^{T −/−} p53^−/− double mutants. The quantification of runx1-positive cells in the AGM was shown in the right panel (mean±s.d., n=60, *P<0.05).

Expression Data

Gene:	runx1
Fish:	WT mir142a^ioz13/ioz13 mir142a^ioz13/ioz13 + MO4-tp53 tp53^zdf1/zdf1; mir142a^ioz13/ioz13
Knockdown Reagent:	MO4-tp53
Anatomical Terms:	trunk ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mir142a^ioz13/ioz13 mir142a^ioz13/ioz13 + MO4-tp53 tp53^zdf1/zdf1; mir142a^ioz13/ioz13
Knockdown Reagent:	MO4-tp53
Observed In:	trunk ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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