FIGURE

Fig. 7

ID

ZDB-FIG-171207-33

Publication

Lu et al., 2015 - Direct regulation of p53 by miR-142a-3p mediates the survival of hematopoietic stem and progenitor cells in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

P53 mediates the decrease of HSPCs in miR-142a-3p mutants. (a) P53 MO-injected 142^T−/− embryos showed the restoration of runx1 expression at 26 and 36 hpf by WISH (left panel). The quantification of runx1-positive cells in the AGM was shown in the right panel (mean±s.d., n=3, *P<0.05). (b) qPCR showed that compared with 142^T−/− at 36 hpf, the decrease in runx1 expression was rescued in 142^{T −/−} embryos injected with p53 MO (mean±s.d., n=3, *P<0.05). (c) WISH showed the expression of runx1 in 142^T+/+ p53^+/+, 142^T−/− p53^+/+ and 142^T−/− p53^−/− embryos at 36 hpf. There was a rescue effect on the decreased runx1 expression in 142^{T −/−} p53^−/− double mutants. The quantification of runx1-positive cells in the AGM was shown in the right panel (mean±s.d., n=60, *P<0.05).

Expression Data

Gene:	runx1
Fish:	WT mir142a^ioz13/ioz13 mir142a^ioz13/ioz13 + MO4-tp53 tp53^zdf1/zdf1; mir142a^ioz13/ioz13
Knockdown Reagent:	MO4-tp53
Anatomical Terms:	trunk ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mir142a^ioz13/ioz13 mir142a^ioz13/ioz13 + MO4-tp53 tp53^zdf1/zdf1; mir142a^ioz13/ioz13
Knockdown Reagent:	MO4-tp53
Observed In:	trunk ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Discov