ZFIN ID: ZDB-FIG-170914-53
Askary et al., 2017 - Genome-wide analysis of facial skeletal regionalization in zebrafish. Development (Cambridge, England)   144(16):2994-3005 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25

Fig. 2

Arch expression of predicted domain-specific genes. Whole-mount fluorescent in situ hybridizations for select genes were performed in sox10:GFPCAAX embryos at 36 hpf, with anti-GFP staining (green) showing CNCCs of the mandibular and hyoid arches. (A) foxf1, foxf2a, fzd9b, smad6a and skp2 are expressed in ventral domains of both arches, pitx1 only in the ventral mandibular arch, and sema3bl and twist1b in ventral and dorsal CNCCs. (B) fgfbp2a and shox are expressed in ventral-intermediate CNCCs, stmn1a in ventral-intermediate and dorsal CNCCs, tmem107I in ventral mandibular and posterior dorsal hyoid arches, and her6 in a more dorsal domain. (C) fsta, ctgfb, foxd1, emx2 and igfbp5b show specific intermediate domain expression; igfbp5b is also expressed in arch mesoderm. (D) cdh11, cd248a, kctd15a, pou3f3a, pou3f3b and postnb are expressed in dorsal CNCCs, sfrp2 in dorsal arch mesoderm, rassf10a in epithelia, and osr2 between the dorsal first arch and eye. Arrows point to expression in predicted domains, and arrowheads to other arch domains. Single optical sections are shown. Scale bar: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cd248a el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
cdh11 el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
ccn2b el375Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
EGFP el375Tg standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
emx2 el375Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
fgfbp2a el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
foxd1 el375Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
foxf1 el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
foxf2a el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
fsta el375Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
fzd9b el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
her6 el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
igfbp5b el375Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
kctd15a el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
osr2 el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
pitx1 el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
postnb el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
pou3f3a el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
pou3f3b el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
rassf10a el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
sema3bl el375Tg standard conditions Prim-25 pharyngeal arch 2 ventral-most region ISH
sfrp2 el375Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
shox el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
skp2 el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
smad6a el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
stmn1a el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
tmem107l el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
twist1b el375Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development