FIGURE

Fig. 5

ID

ZDB-FIG-170824-8

Publication

Roberson et al., 2017 - Convergence of signaling pathways underlying habenular formation and axonal outgrowth

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Fig. 5

Wnt signaling defines the domain of Fgf signaling in the dorsal diencephalon. (A-C′) At 35 hpf, the domain of fgf8a expression just rostral to the developing dHb is expanded in wls mutants. (D,D′) Inhibition of Wnt signaling at 36 hpf by heat shock activation of Tg(hsp70:dkk1-GFP) also expands fgf8a expression at 48 hpf. (E-H′) The expression domains of (E,F) dusp6 and (G,H) etv5b, two targets of Fgf signaling, are enlarged in (E′,F′,G′,H′) wls mutants (compare brackets). (I-L) Expression of dbx1b (arrowheads) in the (I) WT dorsal diencephalon is reduced in (J) fgf8a and (K) wls mutants, and absent in (L) fgf8a;wls double mutants. Dorsal (A,C,D,E,G) and lateral (B,F,H,I-L) views are shown. Refer to Table 1 for numerical values. Sibling WT and homozygous wls mutant embryos were distinguished by the presence or absence of wls transcripts (Kuan et al., 2015). Scale bars: 50 µm.

Expression Data

Genes:	dbx1b dusp6 etv5b fgf8a wls
Fish:	WT fgf8a^{ti282a/ti282a} wls^c186/c186 w32Tg fgf8a^{ti282a/ti282a}; wls^c186/c186
Condition:	heat shock
Anatomical Terms:	diencephalon diencephalon dorsal region
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgf8a^{ti282a/ti282a} wls^c186/c186 w32Tg fgf8a^{ti282a/ti282a}; wls^c186/c186
Condition:	heat shock
Observed In:	diencephalon diencephalon dorsal region dorsal habenular nucleus
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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