FIGURE

Fig. 7

ID

ZDB-FIG-170824-10

Publication

Roberson et al., 2017 - Convergence of signaling pathways underlying habenular formation and axonal outgrowth

Other Figures

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Fig. 7

Chemokine signaling directs outgrowth of dHb axons. (A) In 5 dpf WT larvae, TgBAC(gng8:CAAX-GFP) labels neurons in the left and right dHb and their efferent axons in the FR, which terminate at the midbrain IPN. (B-D) Axonal morphology of 5 dpf (B) cxcr4b, (C) cxcl12a and (D) wls mutants falls into three general phenotypic classes: in Class I, the majority of axons fasciculate normally and project posteriorly; in Class II, some ectopic axons extend anterior to the dHb; and in Class III, the majority of axons grow anteriorly and fasciculate as a thick midline bundle. Asterisks indicate the dHb. Scale bar: 50 µm. (E) Percentage of WT siblings (blue, n=41) and cxcr4b (red, n=35), cxcl12a (green, n=30) and wls (purple, n=43) mutants with Class I, II or III axonal morphology.

Expression Data

Gene:	GFP
Fish:	c375Tg
Anatomical Terms:	dorsal habenular nucleus fasciculus retroflexus
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cxcr4b^{t26035/t26035}; c375Tg wls^c186/c186; c375Tg
Observed In:	axon guidance dorsal habenular nucleus dorsal habenular nucleus axon dorsal habenular nucleus neuron projection
Stage:	Day 5

Phenotype Detail

Acknowledgments

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