Fgf signaling regulates expression domains of chemokine pathway components. (A-F) The (A-C) cxcl12a and (D-F) cxcl12b genes are transcribed in regions adjacent to the dHb at 35?hpf, as shown by fluorescence in situ hybridization and immunolabeling for GFP in TgBAC(dbx1b:GFP) embryos. (G-H?) cxcl12b and (I-J?) ackr3b expression domains (brackets) are expanded in wls mutants. In fgf8a mutants, (K-L?) cxcl12b and (M-N?) ackr3b dorsal diencephalic expression domains (arrowheads) are reduced. Although the cxcl12b expression domain is reduced in (P) fgf8a mutants and expanded in (Q) wls mutants, (R) fgf8a;wls double mutants show a pattern more similar to (O) WT. Between 24 and 48?hpf, WT and wls mutant siblings were treated with either (S,S?) 0.3% DMSO, (T,T?) 18?µM SU5402+0.3% DMSO, or (U,U?) 24?µM SU5402+0.3% DMSO. Following treatment with 24?µM SU5402, cxcl12b expression in wls mutants is restored to the WT pattern (brackets). Scale bar: 30?µm. Refer to Table 1 for numerical values. Dorsal (A-G?,I,I?,K,K?,M,M?,S-U?) and lateral (H,H?,J,J?,L,L?,N,N?,O-R) views are shown. Scale bars: 50?µm.
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