ZFIN ID: ZDB-FIG-170719-9
García-Valtanen et al., 2017 - Zebra Fish Lacking Adaptive Immunity Acquire an Antiviral Alert State Characterized by Upregulated Gene Expression of Apoptosis, Multigene Families, and Interferon-Related Genes. Frontiers in immunology   8:121 Full text @ Front Immunol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-5 to Adult

Fig. 1

Selected gene sets (sGS) were downregulated in rag1−/− egg embryos but were similar in early hatched larvae and both showed high mortalities when infected by spring viremia carp virus (SVCV). (A) Expression folds in embryo eggs 1 day after fertilization (n = 60). Reverse transcriptase and quantitative polymerase chain reaction data were first normalized by the formula, expression of each gene/expression of ef1a. Differential folds were then calculated by the formula, normalized expression of each rag1−/− gene/normalized expression of each rag1+/+ gene. Tbk1 fold was lower than 2−6 (not shown). Tnfa and nklysin were not done. Red dashed line, onefold boundary. *Statistically different from onefold with p < 0.05 by the t-test. (B) Expression folds in hatched larvae 3 days after fertilization (n = 45) calculated as in (A). (C) Kaplan–Meier survival curves of naïve rag1−/− (n = 90) and rag+/+ (n = 90) recently hatched larvae after bath infection in 104 pfu of SVCV per ml (n = 2) at 22°C (optimal replication for SVCV). Differences between rag1−/− and rag+/+ survival curves of SVCV-infected larvae as determined by the Gehan–Breslow–Wilcoxon test were significant with p < 0.05 (*). Solid lines, rag1−/− larvae. Dashed lines, rag+/+ larvae.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cd4-1 rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
cd8a rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
defbl2 rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
ifng1r rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
ighm rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
il1b rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
irf3 rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
mxc rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
tbk1 rag1t26683/t26683 standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
tnfa rag1t26683/t26683 standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT viral treatment by exposure to environment: Carp sprivivirus Adult whole organism decreased life span, abnormal
rag1t26683/t26683 standard conditions Prim-5 whole organism cd4-1 expression decreased amount, abnormal
Prim-5 whole organism cd8a expression decreased amount, abnormal
Prim-5 whole organism defbl2 expression decreased amount, abnormal
Prim-5 whole organism ifng1r expression decreased amount, abnormal
Prim-5 whole organism ighm expression decreased amount, abnormal
Prim-5 whole organism il1b expression decreased amount, abnormal
Prim-5 whole organism irf3 expression decreased amount, abnormal
Prim-5 whole organism mxc expression decreased amount, abnormal
Prim-5 whole organism tbk1 expression decreased amount, abnormal
Protruding-mouth whole organism defbl2 expression decreased amount, abnormal
Protruding-mouth whole organism ifng1r expression decreased amount, abnormal
Protruding-mouth whole organism ighm expression decreased amount, abnormal
Protruding-mouth whole organism il1b expression decreased amount, abnormal
Protruding-mouth whole organism irf3 expression decreased amount, abnormal
Protruding-mouth whole organism mxc expression decreased amount, abnormal
Protruding-mouth whole organism tbk1 expression decreased amount, abnormal
Protruding-mouth whole organism tnfa expression decreased amount, abnormal
rag1t26683/t26683 viral treatment by exposure to environment: Carp sprivivirus Adult whole organism decreased life span, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in immunology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Immunol