ZFIN ID: ZDB-FIG-160715-14
Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development (Cambridge, England)   143(12):2103-10 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

T cells are absent in mutant line 116 embryos. (A,B) WISH showing that early T cell markers (rag1, rag2 and bcl11b), HSPC markers (cmyb and ikaros) and T cell markers (ccr9a, ccr9b, tcrb2 and tcrd) are absent in the thymus of mutant line 116 at 5dpf. By contrast, thymus epithelial cell marker foxn1 was normal in the thymus of 116 mutants at 5dpf. Red arrowheads indicate the thymus. Numbers at bottom right indicate the number of embryos with similar staining pattern among all embryos examined.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bcl11ba AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
ccr9a AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
ccr9b AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
foxn1 AB standard conditions Day 5 thymic epithelium ISH
crcphkz8/hkz8 standard conditions Day 5 thymic epithelium ISH
ikzf1 AB standard conditions Day 5 thymus hematopoietic multipotent progenitor cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
linc-tcrb AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
myb AB standard conditions Day 5 thymus hematopoietic multipotent progenitor cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
rag1 AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
rag2 AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
trdc AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
crcphkz8/hkz8 standard conditions Day 5 thymus bcl11ba expression absent, abnormal
Day 5 thymus ccr9a expression absent, abnormal
Day 5 thymus ccr9b expression absent, abnormal
Day 5 thymus ikzf1 expression absent, abnormal
Day 5 thymus myb expression absent, abnormal
Day 5 thymus rag1 expression absent, abnormal
Day 5 thymus rag2 expression absent, abnormal
Day 5 thymus trdc expression absent, abnormal
Day 5 thymus hematopoietic multipotent progenitor cell absent, abnormal
Day 5 thymus linc-tcrb expression absent, abnormal
Day 5 thymus T cell absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development