ZFIN ID: ZDB-FIG-160715-15
Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development (Cambridge, England)   143(12):2103-10 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 2

Lack of Rpc9 is responsible for the phenotype of mutant line 116 embryos. (A) Schematic of positional cloning. a-g indicate the locations of polymorphic primers distributed between 29,708,538 to 30,321,343bp of chromosome 21 and the ratios of recombination out of total 2208 embryos in each position. Genes scattered in this region are marked and rpc9 (in red) is adjacent to the polymorphic marker d. Note that the chromosomal locations and sequences of these primers are listed in Table S3. (B) Gene sequencing identified a transversion of T>A in the second exon (exon 2) of rpc9, which formed a pre-mature stop code in the coding region of rpc9 in mutant line 116 embryos. (C) Western blot showed that the protein level of Rpc9 was greatly decreased in mutants at 4dpf. (D) Injection of the rpc9 overexpression plasmid driven by hsp70 promoter (hsp70-rpc9-egfp) exerted a full rescue effect of rag1. Red arrowheads in D indicate the thymus.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
crcp Ab1-crcp AB standard conditions Day 4 whole organism WB
Ab1-crcp crcphkz8/hkz8 standard conditions Day 4 whole organism WB
rag1 AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
crcphkz8/hkz8 standard conditions Day 4 whole organism crcp expression decreased amount, abnormal
Day 5 thymus rag1 expression absent, abnormal
Day 5 thymus T cell absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development