ZFIN ID: ZDB-FIG-160715-18
Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development (Cambridge, England)   143(12):2103-10 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 5

Knockdown of Pol III components polr3h and polr3k leads to HSPC defects. (A) qRT-PCR result shows that, compared with control embryos, products of Pol III (pre-tRNAIle, pre-tRNALeu, 5S rRNA and 7SL RNA) are all significantly decreased in both polr3h and polr3k morphants (5dpf). 18S rRNA was used as internal control. (B) WISH result showing that HSPC marker (cmyb) and differentiated hematopoietic cell lineage markers (gata1, pu.1 and rag1) are all decreased in polr3h and polr3k morphants at 5dpf. (C) qRT-PCR result reveals that cmyb, gata1 and pu.1 are significantly decreased in polr3h and polr3k morphants at 5dpf. β-actin was used as internal control. Note that arrowheads in B mark the CHT or the thymus. Values are meanĀ±s.d. **P<0.01, ***P<0.001, Student′s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a AB control Day 5 hematopoietic system posterior region ISH
AB + MO1-polr3h standard conditions Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
AB + MO1-polr3k standard conditions Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
myb AB control Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
AB + MO1-polr3h standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
AB + MO1-polr3k standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
rag1 AB control Day 5 thymus ISH
AB + MO1-polr3h standard conditions Day 5 Not Detected thymus ISH
AB + MO1-polr3k standard conditions Day 5 Not Detected thymus ISH
spi1b AB control Day 5 hematopoietic system posterior region ISH
AB + MO1-polr3h standard conditions Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
AB + MO1-polr3k standard conditions Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-polr3h standard conditions Day 5 hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal
Day 5 hematopoietic multipotent progenitor cell decreased amount, abnormal
Day 5 hematopoietic system posterior region gata1a expression decreased amount, abnormal
Day 5 hematopoietic system posterior region myb expression decreased amount, abnormal
Day 5 hematopoietic system posterior region spi1b expression decreased amount, abnormal
Day 5 thymus rag1 expression absent, abnormal
Day 5 thymus T cell absent, abnormal
Day 5 whole organism gata1a expression decreased amount, abnormal
Day 5 whole organism myb expression decreased amount, abnormal
Day 5 whole organism spi1b expression decreased amount, abnormal
AB + MO1-polr3k standard conditions Day 5 hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal
Day 5 hematopoietic multipotent progenitor cell decreased amount, abnormal
Day 5 hematopoietic system posterior region gata1a expression decreased amount, abnormal
Day 5 hematopoietic system posterior region myb expression decreased amount, abnormal
Day 5 hematopoietic system posterior region spi1b expression decreased amount, abnormal
Day 5 thymus rag1 expression absent, abnormal
Day 5 thymus T cell absent, abnormal
Day 5 whole organism gata1a expression decreased amount, abnormal
Day 5 whole organism myb expression decreased amount, abnormal
Day 5 whole organism spi1b expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development