FIGURE

Fig. 3

ID

ZDB-FIG-160715-16

Publication

Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish

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Fig. 3

HSPCs are impaired in rpc9^-/- embryos. (A) WISH showing that ikaros, at 3dpf, and tcrd and tcrb2, at 4dpf, were decreased in the thymus of rpc9^-/- embryos. (B) WISH demonstrates that runx1 expression is not decreased in the AGM region of rpc9^-/- embryos at 32 h post-fertilization (hpf) and cmyb clearly begins to decrease from 3dpf in the CHT region of rpc9^-/- embryos. (C) qRT-PCR with the cDNA reverse transcribed from total RNA of posterior trunk region reveals that cmyb expression is significantly decreased in rpc9^-/- embryos from 3 to 5dpf. β-actin was used as internal control (mean±s.d., n=3, **P<0.01, ***P<0.001). (D) Expression pattern of rpc9 in the CHT region at 3 and 4dpf. Note that arrowheads in A mark the thymus, whereas arrowheads in B,D mark the CHT. Values are mean±s.e.m.; **P<0.01, ***P<0.001, Student′s t-test. ns, not significant.

Expression Data

Genes:	crcp ikzf1 linc-tcrb myb runx1 trdc (all 6)
Fish:	AB crcp^hkz8/hkz8
Anatomical Terms:	hematopoietic system posterior region intermediate cell mass of mesoderm thymus thymus hematopoietic multipotent progenitor cell thymus T cell trunk posterior region ventral wall of dorsal aorta (all 7)
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	crcp^hkz8/hkz8
Observed In:	hematopoietic system posterior region thymus trunk posterior region
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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