ZFIN ID: ZDB-FIG-160715-16
Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development (Cambridge, England)   143(12):2103-10 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 3

HSPCs are impaired in rpc9-/- embryos. (A) WISH showing that ikaros, at 3dpf, and tcrd and tcrb2, at 4dpf, were decreased in the thymus of rpc9-/- embryos. (B) WISH demonstrates that runx1 expression is not decreased in the AGM region of rpc9-/- embryos at 32 h post-fertilization (hpf) and cmyb clearly begins to decrease from 3dpf in the CHT region of rpc9-/- embryos. (C) qRT-PCR with the cDNA reverse transcribed from total RNA of posterior trunk region reveals that cmyb expression is significantly decreased in rpc9-/- embryos from 3 to 5dpf. β-actin was used as internal control (mean±s.d., n=3, **P<0.01, ***P<0.001). (D) Expression pattern of rpc9 in the CHT region at 3 and 4dpf. Note that arrowheads in A mark the thymus, whereas arrowheads in B,D mark the CHT. Values are mean±s.e.m.; **P<0.01, ***P<0.001, Student′s t-test. ns, not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crcp AB standard conditions Protruding-mouth hematopoietic system posterior region ISH
Day 4 hematopoietic system posterior region ISH
ikzf1 AB standard conditions Protruding-mouth thymus hematopoietic multipotent progenitor cell ISH
crcphkz8/hkz8 standard conditions Protruding-mouth thymus ISH
linc-tcrb AB standard conditions Day 4 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 4 thymus ISH
myb AB standard conditions Long-pec intermediate cell mass of mesoderm ISH
Protruding-mouth hematopoietic system posterior region ISH
Day 4 hematopoietic system posterior region ISH
Day 5 hematopoietic system posterior region ISH
crcphkz8/hkz8 standard conditions Long-pec intermediate cell mass of mesoderm ISH
Protruding-mouth hematopoietic system posterior region ISH
Protruding-mouth trunk posterior region RTPCR
Day 4 hematopoietic system posterior region ISH
Day 4 trunk posterior region RTPCR
Day 5 hematopoietic system posterior region ISH
Day 5 trunk posterior region RTPCR
runx1 AB standard conditions Prim-5 ventral wall of dorsal aorta ISH
Prim-15 ventral wall of dorsal aorta ISH
crcphkz8/hkz8 standard conditions Prim-5 ventral wall of dorsal aorta ISH
Prim-15 ventral wall of dorsal aorta ISH
trdc AB standard conditions Day 4 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 4 thymus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
crcphkz8/hkz8 standard conditions Protruding-mouth hematopoietic system posterior region myb expression decreased amount, abnormal
Protruding-mouth thymus ikzf1 expression decreased amount, abnormal
Protruding-mouth trunk posterior region myb expression decreased amount, abnormal
Day 4 hematopoietic system posterior region myb expression decreased amount, abnormal
Day 4 thymus trdc expression decreased amount, abnormal
Day 4 thymus linc-tcrb expression decreased amount, abnormal
Day 4 trunk posterior region myb expression decreased amount, abnormal
Day 5 hematopoietic system posterior region myb expression decreased amount, abnormal
Day 5 trunk posterior region myb expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development