ZFIN ID: ZDB-FIG-160715-19
Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development (Cambridge, England)   143(12):2103-10 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 6

P53 mediates the regulation of Rpc9 in HSPC survival. (A) WISH result reveals that p53 is specifically increased in the CHT region of rpc9-/- embryos at 3 and 4dpf. (B) Western blot shows that, compared with rpc9+/+ embryos, the protein level of P53 was greatly increased in rpc9-/- embryos at 4dpf. (C) The quantitative result of the western blot in B. (D) The absence of rag1 in the thymus of rpc9-/- embryos, at 5dpf, can be rescued by loss of p53. (E) The absence of cmyb in the CHT region of rpc9-/- embryos at 5dpf can be rescued by p53 deficiency. (F) qRT-PCR result reveals that the decrease of cmyb in the CHT region of rpc9-/- embryos at 5dpf can be rescued by loss of p53. Note that arrowheads in A,E mark the CHT, whereas arrowheads in D mark the thymus. Values are meanĀ±s.e.m. ***P<0.001, Student′s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myb AB standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
crcphkz8/hkz8 standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
crcphkz8/hkz8; tp53zdf1/zdf1 standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
tp53zdf1/zdf1 standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
rag1 AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
crcphkz8/hkz8; tp53zdf1/zdf1 standard conditions Day 5 thymus ISH
tp53zdf1/zdf1 standard conditions Day 5 thymus T cell ISH
tp53 AB standard conditions Protruding-mouth hematopoietic system posterior region ISH
Day 4 hematopoietic system posterior region ISH
crcphkz8/hkz8 standard conditions Protruding-mouth hematopoietic system posterior region ISH
Day 4 hematopoietic system posterior region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab13-tp53 WB crcphkz8/hkz8 standard conditions Day 4 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
crcphkz8/hkz8 standard conditions Protruding-mouth hematopoietic system posterior region tp53 expression increased amount, abnormal
Day 4 hematopoietic system posterior region tp53 expression increased amount, abnormal
Day 4 whole organism ab13-tp53 labeling increased amount, abnormal
Day 5 hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal
Day 5 hematopoietic system posterior region myb expression decreased amount, abnormal
Day 5 thymus rag1 expression absent, abnormal
Day 5 whole organism myb expression decreased amount, abnormal
crcphkz8/hkz8; tp53zdf1/zdf1 standard conditions Day 5 hematopoietic multipotent progenitor cell myb expression amount, ameliorated
Day 5 hematopoietic system posterior region myb expression amount, ameliorated
Day 5 thymus rag1 expression amount, ameliorated
Day 5 whole organism myb expression amount, ameliorated
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development