ZFIN ID: ZDB-FIG-160715-19
Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development (Cambridge, England)   143(12):2103-10 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 6

P53 mediates the regulation of Rpc9 in HSPC survival. (A) WISH result reveals that p53 is specifically increased in the CHT region of rpc9-/- embryos at 3 and 4dpf. (B) Western blot shows that, compared with rpc9+/+ embryos, the protein level of P53 was greatly increased in rpc9-/- embryos at 4dpf. (C) The quantitative result of the western blot in B. (D) The absence of rag1 in the thymus of rpc9-/- embryos, at 5dpf, can be rescued by loss of p53. (E) The absence of cmyb in the CHT region of rpc9-/- embryos at 5dpf can be rescued by p53 deficiency. (F) qRT-PCR result reveals that the decrease of cmyb in the CHT region of rpc9-/- embryos at 5dpf can be rescued by loss of p53. Note that arrowheads in A,E mark the CHT, whereas arrowheads in D mark the thymus. Values are meanĀ±s.e.m. ***P<0.001, Student′s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myb AB standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
crcphkz8/hkz8 standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
crcphkz8/hkz8; tp53zdf1/zdf1 standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
Day 5 whole organism RTPCR
tp53zdf1/zdf1 standard conditions Day 5 hematopoietic multipotent progenitor cell ISH
Day 5 hematopoietic system posterior region ISH
rag1 AB standard conditions Day 5 thymus T cell ISH
crcphkz8/hkz8 standard conditions Day 5 Not Detected thymus ISH
crcphkz8/hkz8; tp53zdf1/zdf1 standard conditions Day 5 thymus ISH
tp53zdf1/zdf1 standard conditions Day 5 thymus T cell ISH
tp53 AB standard conditions Protruding-mouth hematopoietic system posterior region ISH
Day 4 hematopoietic system posterior region ISH
crcphkz8/hkz8 standard conditions Protruding-mouth hematopoietic system posterior region ISH
Day 4 hematopoietic system posterior region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab13-tp53 WB crcphkz8/hkz8 standard conditions Day 4 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
crcphkz8/hkz8 standard conditions Protruding-mouth hematopoietic system posterior region tp53 expression increased amount, abnormal
Day 4 hematopoietic system posterior region tp53 expression increased amount, abnormal
Day 4 whole organism ab13-tp53 labeling increased amount, abnormal
Day 5 hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal
Day 5 hematopoietic system posterior region myb expression decreased amount, abnormal
Day 5 thymus rag1 expression absent, abnormal
Day 5 whole organism myb expression decreased amount, abnormal
crcphkz8/hkz8; tp53zdf1/zdf1 standard conditions Day 5 hematopoietic multipotent progenitor cell myb expression amount, ameliorated
Day 5 hematopoietic system posterior region myb expression amount, ameliorated
Day 5 thymus rag1 expression amount, ameliorated
Day 5 whole organism myb expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development