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ZFIN ID: ZDB-FIG-150811-10
Moreno-Ayala et al., 2015 - PIAS-like protein Zimp7 is required for the restriction of the Zebrafish organizer and mesoderm development. Developmental Biology   403(1):89-100 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 30%-epiboly to 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Shield

Fig. 5

Zimp7 restricts organizer gene expression. Dorsal and ventral gene expression in embryos at shield (A–N), 5 hpf (O–Q) and 3-somite stage (R–T). Embryos were hybridized to specific marker genes boz (A and B), gsc (C–E), flh (F–H), vox (I–K), vent (L–N), ntl (O–Q) and hgg1 (R–T). The injected reagents are indicated at the left. boz, lateral views, dorsal to the right; gsc, and flh dorsal views with the animal pole oriented toward the top; vox, vent and ntl, animal pole views with dorsal to the right; hgg1, anterior view, dorsal side to the top. Arrows in I–N indicate the angle of marginal expression of ventral genes. An asterisk indicates the ectopic expression of vent in the ectoderm. U and V, graphs showing average values obtained from a number of embryos (indicated at the bottom of each bar) as the ones shown in A–T. U, the relative differences in mean expression area in MO and mRNA injected embryos compared to control embryos (horizontal line normalized to one) for the dorsal genes and hgg1. V, the average negative expression angle in MO and mRNA injected embryos compared to control embryos for the ventral genes vox and vent. The number of embryos with given appearance for ntl (Q) is 81% (n=21). zimp7 morphants display a significant increase in all dorsal genes as well as in the organizer-induced gene hgg1 and a reduction in ventral gene expression. Opposite effects are observed when zimp7 is overexpressed. Notably, besides an increased marginal angle in ventral genes, there is augmented ectodermal expression. Np<0.05; **p<0.01; ***p<0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT control 1-4 somites prechordal plate ISH
WT + MO1-zmiz2 standard conditions 1-4 somites prechordal plate ISH
dharma WT control Shield shield ISH
WT + MO1-zmiz2 standard conditions Shield shield ISH
gsc WT control Shield shield ISH
WT + MO1-zmiz2 standard conditions Shield shield ISH
noto WT control Shield shield ISH
WT + MO1-zmiz2 standard conditions Shield shield ISH
tbxta WT control 30%-epiboly margin ISH
WT + MO1-zmiz2 standard conditions 30%-epiboly margin ISH
vent WT control Shield margin ISH
WT + MO1-zmiz2 standard conditions Shield margin ISH
vox WT control Shield margin ISH
WT + MO1-zmiz2 standard conditions Shield margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-zmiz2 standard conditions Shield dorsal/ventral pattern formation process quality, abnormal
Shield shield increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 403(1), Moreno-Ayala, R., Schnabel, D., Salas-Vidal, E., Lomelí, H., PIAS-like protein Zimp7 is required for the restriction of the Zebrafish organizer and mesoderm development, 89-100, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.