ZFIN ID: ZDB-FIG-150811-12 |
Genes: | |
---|---|
Fish: | |
Knockdown Reagent: | |
Anatomical Terms: | |
Stage Range: | Shield to 1-4 somites |
Fig. 7 Co-injection of zimp7-sp MO and mRNA restores the normal level of expression of markers in embryos. (A) Anterior views of 3-somite stage embryos with dorsal toward the top. Expression of hgg1 is shown for control embryos, zimp7-sp MO injected embryos and embryos co-injected with zimp7-sp MO plus the indicated amounts of zimp7 mRNA. (B) Expression of gsc at shield stage in control embryos, zimp7-sp MO injected embryos and embryos co-injected with zimp7-sp MO plus the indicated amounts of zimp7 mRNA. Dorsal views. (C and D) For both markers the expression area was measured for a group of embryos (numbers indicated at the bottom of each bar) and the average area was plotted for each condition. Statistical analysis indicated a significant rescue of the normal level of expression for both markers. Bars represent standard deviation. **p<0.01; ***p<0.001. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
---|---|---|---|---|---|---|
ctslb | WT | control | 1-4 somites | prechordal plate | ISH | |
WT + MO1-zmiz2 | standard conditions | 1-4 somites | prechordal plate | ISH | ||
gsc | WT | control | Shield | shield | ISH | |
WT + MO1-zmiz2 | standard conditions | Shield | shield | ISH |
Acknowledgments: |
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Biology, 403(1), Moreno-Ayala, R., Schnabel, D., Salas-Vidal, E., LomelĂ, H., PIAS-like protein Zimp7 is required for the restriction of the Zebrafish organizer and mesoderm development, 89-100, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol. |
---|