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ZFIN ID: ZDB-FIG-150629-11
Nagendran et al., 2015 - Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages. Development (Cambridge, England)   142(2):320-30 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-15 to Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 2 Gain of Wnt signalling results in acquisition of stretched cell morphology in the median fin fold. Bright-field images of wild-type sibling (A), apc mutant (B), DMSO- (C) and 0.5 mM BIO-treated (D) embryos. (E-H′) β-Catenin-DAPI overlays (E-H) and β-catenin staining (E′-H′) in given genetic backgrounds and treatments. (I-L) E-cadherin staining in wild-type siblings (I), apc mutant (J), DMSO control (K) and BIO-treated (L) embryos. (M,N) Comparison of epithelial cell aspect ratio plots between sibling and apc mutant (M) as well as between DMSO and BIO-treated embryos (N). Scale bars: 0.1mm in A-D; 10 μm in E-L.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC TU control Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 epidermal basal stratum nucleus
IHC Prim-15 median fin fold epidermal basal stratum
IHC TU standard conditions Prim-25 epidermal basal stratum cell-cell junction
IHC Prim-25 epidermal basal stratum nucleus
IHC Prim-25 median fin fold epidermal basal stratum
IHC TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 epidermal basal stratum nucleus
IHC Prim-15 median fin fold epidermal basal stratum
IHC apczf134/zf134 standard conditions Prim-25 epidermal basal stratum cell-cell junction
IHC Prim-25 epidermal basal stratum nucleus
IHC Prim-25 median fin fold epidermal basal stratum
Ab2-cdh1 IHC TU control Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 median fin fold epidermal basal stratum
IHC TU standard conditions Prim-25 epidermal basal stratum cell-cell junction
IHC Prim-25 median fin fold epidermal basal stratum
IHC TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 median fin fold epidermal basal stratum
IHC apczf134/zf134 standard conditions Prim-25 epidermal basal stratum cell-cell junction
IHC Prim-25 median fin fold epidermal basal stratum
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-15 embryonic medial fin morphogenesis decreased process quality, abnormal
TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-15 epidermal basal stratum decreased height, abnormal
TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-15 epidermal basal stratum canonical Wnt signaling pathway increased process quality, abnormal
TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-15 epidermal basal stratum ecto-epithelial cell elongated, abnormal
TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-15 epidermal basal stratum epithelial cell morphogenesis decreased process quality, abnormal
apczf134/zf134 standard conditions Prim-25 embryonic medial fin morphogenesis decreased process quality, abnormal
Prim-25 epidermal basal stratum decreased height, abnormal
Prim-25 epidermal basal stratum canonical Wnt signaling pathway increased process quality, abnormal
Prim-25 epidermal basal stratum ecto-epithelial cell elongated, abnormal
Prim-25 epidermal basal stratum epithelial cell morphogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development