PUBLICATION
Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages
- Authors
- Nagendran, M., Arora, P., Gori, P., Mulay, A., Ray, S., Jacob, T., Sonawane, M.
- ID
- ZDB-PUB-141219-13
- Date
- 2015
- Source
- Development (Cambridge, England) 142(2): 320-30 (Journal)
- Registered Authors
- Sonawane, Mahendra
- Keywords
- none
- MeSH Terms
-
- Animals
- Epithelium/embryology*
- Extremities/embryology*
- In Situ Hybridization
- Laminin/metabolism*
- Microscopy, Fluorescence
- Morphogenesis/physiology
- Real-Time Polymerase Chain Reaction
- Wnt Signaling Pathway/physiology*
- Zebrafish/embryology*
- PubMed
- 25519245 Full text @ Development
Citation
Nagendran, M., Arora, P., Gori, P., Mulay, A., Ray, S., Jacob, T., Sonawane, M. (2015) Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages. Development (Cambridge, England). 142(2):320-30.
Abstract
The patterning and morphogenesis of body appendages - such as limbs and fins - is orchestrated by the activities of several developmental pathways. Wnt signalling is essential for the induction of limbs. However, it is unclear whether a canonical Wnt signalling gradient exists and regulates the patterning of epithelium in vertebrate appendages. Using an evolutionarily old appendage - the median fin in zebrafish - as a model, we show that the fin epithelium exhibits graded changes in cellular morphology along the proximo-distal axis. This epithelial pattern is strictly correlated with the gradient of canonical Wnt signalling activity. By combining genetic analyses with cellular imaging, we show that canonical Wnt signalling regulates epithelial cell morphology by modulating the levels of laminins, which are extracellular matrix components. We have unravelled a hitherto unknown mechanism involved in epithelial patterning, which is also conserved in the pectoral fins - evolutionarily recent appendages that are homologous to tetrapod limbs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping