ZFIN ID: ZDB-FIG-150629-13
Nagendran et al., 2015 - Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages. Development (Cambridge, England)   142(2):320-30 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 4 Canonical Wnt signalling is required persistently within a specific time window to alter cell shapes. (A-D′) β-Catenin-DAPI overlays (A-D) and β-catenin staining (A′-D′) in DMSO control (A,A′) and embryos treated with the mentioned doses of BIO drug (B-D,B′-D′). (A"-D") Comparison of nuclear β-catenin levels (A"-D") and aspect ratio plots (A′′′-D′′′) for DMSO control and embryos treated with the mentioned doses of BIO. (E-H) E-cadherin staining in embryos treated with DMSO (E) and with 0.5 mM BIO at 16 (F), 24 (G) and 30 (H) hpf. (I,J,L,M) Cell-shape analysis in DMSO control (I,L) and embryos treated with BIO from 16 hpf continuously and analysed at 30 (J) and at 36 (M) hpf. (K,N) Embryos in which the drug was (K) washed off at 24 hpf and cell shape analysis carried out at 30 hpf or (N) washed off at 30 hpf and analysed at 36 hpf. Scale bars: 10 μm. AU, arbitrary units.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC TU control Prim-5 epidermal basal stratum cell-cell junction
IHC Prim-5 epidermal basal stratum nucleus
IHC Prim-5 median fin fold epidermal basal stratum
IHC TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-5 epidermal basal stratum cell-cell junction
IHC Prim-5 epidermal basal stratum nucleus
IHC Prim-5 median fin fold epidermal basal stratum
Ab2-cdh1 IHC TU control Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 median fin fold epidermal basal stratum
IHC Prim-25 epidermal basal stratum cell-cell junction
IHC Prim-25 median fin fold epidermal basal stratum
IHC TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 median fin fold epidermal basal stratum
IHC Prim-25 epidermal basal stratum cell-cell junction
IHC Prim-25 median fin fold epidermal basal stratum
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment: 6-bromoindirubin-3'-oxime Prim-5 embryonic medial fin morphogenesis decreased process quality, abnormal
Prim-5 epidermal basal stratum decreased height, abnormal
Prim-5 epidermal basal stratum canonical Wnt signaling pathway increased process quality, abnormal
Prim-5 epidermal basal stratum ecto-epithelial cell elongated, abnormal
Prim-5 epidermal basal stratum epithelial cell morphogenesis decreased process quality, abnormal
Prim-15 embryonic medial fin morphogenesis decreased process quality, abnormal
Prim-15 epidermal basal stratum decreased height, abnormal
Prim-15 epidermal basal stratum canonical Wnt signaling pathway increased process quality, abnormal
Prim-15 epidermal basal stratum ecto-epithelial cell elongated, abnormal
Prim-15 epidermal basal stratum epithelial cell morphogenesis decreased process quality, abnormal
Prim-25 embryonic medial fin morphogenesis decreased process quality, abnormal
Prim-25 epidermal basal stratum decreased height, abnormal
Prim-25 epidermal basal stratum canonical Wnt signaling pathway increased process quality, abnormal
Prim-25 epidermal basal stratum ecto-epithelial cell elongated, abnormal
Prim-25 epidermal basal stratum epithelial cell morphogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development