ZFIN ID: ZDB-FIG-150629-10
Nagendran et al., 2015 - Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages. Development (Cambridge, England)   142(2):320-30 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 1 Correlation between cellular pattern and Wnt signalling gradient in the median fin fold epithelium. (A) Bright-field image of 24 hpf wild-type zebrafish embryo. The dotted box represents the region of the fin epithelium imaged by confocal microscopy. (B-G) E-cadherin staining and aspect ratio plots of median fin epithelial cells at 24 (B,E), 30 (C,F) and 36 hpf (D,G) in wild-type embryos. Aspect ratios are plotted against the distance of the cells from the base of the fin fold along PD axis. (H-I′) β-Catenin-DAPI overlays (H,I) and β-catenin staining (H′,I′) and its quantification (H",I") in median fin epithelium at 24 hpf (H-H′) and 30 hpf (I-I′). (J-K′) Tg(7XTCF-Xia.Siam:GFP)ia4 line showing GFP expression in the distal cells of the median fin at lower magnification (J) and at higher magnification (K,K′) along with E-cadherin staining. Arrowheads in H,H′ indicate nuclear β-catenin. Scale bars: 10 μm B-D,H-I′,K,K′ ; 50 μm in J. AU, arbitrary units.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ia4Tg standard conditions Prim-15 median fin fold epidermal basal stratum IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC TU standard conditions Prim-5 epidermal basal stratum cell-cell junction
IHC Prim-5 epidermal basal stratum nucleus
IHC Prim-5 median fin fold epidermal basal stratum
IHC Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 epidermal basal stratum nucleus
IHC Prim-15 median fin fold epidermal basal stratum
Ab2-cdh1 IHC TU standard conditions Prim-5 epidermal basal stratum cell-cell junction
IHC Prim-5 median fin fold epidermal basal stratum
IHC Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 median fin fold epidermal basal stratum
IHC Prim-25 epidermal basal stratum cell-cell junction
IHC Prim-25 median fin fold epidermal basal stratum
IHC ia4Tg standard conditions Prim-15 epidermal basal stratum cell-cell junction
IHC Prim-15 median fin fold epidermal basal stratum
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development