header logo image header logo text
Downloads Login
General Information
ZFIN ID: ZDB-FIG-150629-17
Nagendran et al., 2015 - Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages. Development (Cambridge, England)   142(2):320-30 Full text @ Development
Anatomical Terms:
Stage Range: Long-pec to Pec-fin
Observed In:
Stage: Pec-fin

Fig. 8 Mechanisms patterning median fin fold epithelium are also conserved in the pectoral fin. Bright-field image (A) of 5dpf wild-type pectoral fin. β-Catenin and DAPI-stained pectoral fin (B) and its digitally zoomed (B′) region (box in B). Arrow in B′ indicates stretched epithelial cells at the distal end, whereas arrowhead indicates polygonal cells. (C) Maximum intensity projections of wild-type larval pectoral fin stained for laminin. Confocal images showing E-cadherin staining in DMSO- (D) or BIO-treated (E) larval pectoral fins. (F) Comparison of aspect ratios of pectoral fin epithelial cells in DMSO and BIO-treated larvae. (G) Model showing the regulation of epithelial patterning in zebrafish fin-fold epithelium by the canonical Wnt signalling gradient. A gradient of canonical Wnt activity exists along the PD axis of the fin fold. The gain- and loss-of-function analyses reveal that the extent of canonical Wnt signalling regulates the expression of the ECM component laminin α5, which directly regulates cell shapes by interacting with integrin α3. Scale bars: 10 μm in B′,D,E; 50 μm in B,C. AR, aspect ratio.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC TU standard conditions Long-pec pectoral fin cell-cell junction
IHC Long-pec pectoral fin distal region
IHC Long-pec pectoral fin nucleus
Ab1-lama1 IHC TU standard conditions Pec-fin pectoral fin
Ab2-cdh1 IHC TU control Pec-fin pectoral fin cell-cell junction
IHC TU chemical treatment: 6-bromoindirubin-3'-oxime Pec-fin pectoral fin cell-cell junction
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment: 6-bromoindirubin-3'-oxime Pec-fin embryonic pectoral fin morphogenesis decreased process quality, abnormal
Pec-fin pectoral fin fold decreased size, abnormal
Pec-fin pectoral fin fold ecto-epithelial cell elongated, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development