FIGURE

Fig. 8

ID

ZDB-FIG-150615-8

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Photo-Morpholinos reduce apical domain size. (A–D) Transverse cryosections of 4 dpf retinas were stained with phalloidin to label actin (red) and zpr-1 to label double cones (green). Sections were also counterstained with DAPI (blue). (E) Quantification of apical domain as a percentage of total photoreceptor inner segment length. All values are plotted as a percentage of n/m, as defined in the text. Wild type = 38%; pard3-pard3pMO morphants = 16%; prkci-prkcipMO morphants = 9.72%; prkc-prkcpMO was non-detectable. Blue error bars indicate the mean ± standard deviation. ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer. Scale bar = 20 μm.

Expression Data

Antibody:	zpr-1
Fish:	AB/EKW AB/EKW + MO1-pard3ab AB/EKW + MO1-prkci AB/EKW + MO1-prkci + MO1-prkcz
Knockdown Reagents:	MO1-pard3ab MO1-prkci MO1-prkcz
Anatomical Term:	retinal cone cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/EKW + MO1-pard3ab AB/EKW + MO1-prkci AB/EKW + MO1-prkci + MO1-prkcz
Knockdown Reagents:	MO1-pard3ab MO1-prkci MO1-prkcz
Observed In:	camera-type eye photoreceptor cell differentiation eye photoreceptor cell apical region photoreceptor cell morphogenesis retina retinal cone cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One