|ZFIN ID: ZDB-FIG-150615-6|
Fig. 6 Connecting cilia are reduced in pard3 morphants. (A–C) Transverse cryosections were stained for Ift88 (left, red) and acetylated tubulin (middle, green) to label cilia in 5 dpf retinas. Merged images are shown in right panels. Ift88 localizes to the connecting cilia emanating from the apical surface of photoreceptors (arrows). Staining is also seen in connecting cilia of UV cones (arrowheads), which are tiered below the other cones. Sections were also counterstained with DAPI (blue). ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer. Scale bar = 10 μm.
|Ab1-ift88||AB/EKW||standard conditions||Day 5||IHC|
|Ab1-ift88||AB/EKW + MO1-pard3ab||standard conditions||Day 5||IHC|
|IHC||AB/EKW||standard conditions||Day 5|
|IHC||AB/EKW + MO1-pard3ab||standard conditions||Day 5|
|AB/EKW + MO1-pard3ab||standard conditions||Day 5||eye photoreceptor cell has fewer parts of type eye photoreceptor cell photoreceptor connecting cilium, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One|