ZFIN ID: ZDB-FIG-150615-6
Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors. PLoS One   9:e104661 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 6 Connecting cilia are reduced in pard3 morphants. (A–C) Transverse cryosections were stained for Ift88 (left, red) and acetylated tubulin (middle, green) to label cilia in 5 dpf retinas. Merged images are shown in right panels. Ift88 localizes to the connecting cilia emanating from the apical surface of photoreceptors (arrows). Staining is also seen in connecting cilia of UV cones (arrowheads), which are tiered below the other cones. Sections were also counterstained with DAPI (blue). ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer. Scale bar = 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ift88 Ab1-ift88 AB/EKW control Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
Ab1-ift88 AB/EKW standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
Ab1-ift88 AB/EKW + MO1-pard3ab standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC AB/EKW control Day 5 eye photoreceptor cell photoreceptor connecting cilium
IHC AB/EKW standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium
IHC AB/EKW + MO1-pard3ab standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium
Phenotype Details
Fish Conditions Stage Phenotype
AB/EKW + MO1-pard3ab standard conditions Day 5 eye photoreceptor cell has fewer parts of type eye photoreceptor cell photoreceptor connecting cilium, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One