FIGURE

Fig. 7

ID

ZDB-FIG-150615-7

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

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Fig. 7

Rhodopsin is mislocalized in pard3 morphants. (A) Immunohistochemistry was performed on transverse cryosections through the retina of 5 dpf wild-type larvae. Left most panels show phalloidin staining to label actin (red). Rhodopsin immunoreactivity (green) is shown in middle panels. (D?F) In pard3 morphants, outer segments appear slightly shorter and opsin staining within the inner segment is observed (bracket). (G?I) Rhodopsin localizes normally in control morphants. Sections were also counterstained with DAPI (blue). ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer. Scale bar = 20 μm.

Expression Data

Gene:	rho
Antibody:	Ab1-rho
Fish:	AB/EKW AB/EKW + MO1-pard3ab
Knockdown Reagent:	MO1-pard3ab
Anatomical Terms:	eye photoreceptor cell photoreceptor inner segment eye photoreceptor cell photoreceptor outer segment
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/EKW + MO1-pard3ab
Knockdown Reagent:	MO1-pard3ab
Observed In:	photoreceptor cell photoreceptor inner segment photoreceptor cell photoreceptor outer segment
Stage:	Day 5

Phenotype Detail

Acknowledgments

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