ZFIN ID: ZDB-FIG-150615-1
Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors. PLoS One   9:e104661 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5

Fig. 1 Spatial distribution of cell junction proteins and cilia in 5 dpf zebrafish. (A–E) Left-most panels show transverse cryosections stained with phalloidin (red) to label actin in the adherens junctions at the outer limiting membrane (OLM) and the outer plexiform layer (OPL). The middle panels show staining for determinants of apico-basal polarity (green), while the right-most panels show the merged images. (A1–A3) PrkCi/z immunoreactivity. (B1–B3) Pard3 immunoreactivity localizes to the OPL (arrow) and at cell junctions (arrowhead). (C1–C3) ZO-1 staining at the OLM. (D1–D3) Ift88 immunoreactivity stains cilia. (E1–E3) Acetylated tubulin immunoreactivity was seen in cilia and neural processes throughout the inner nuclear layer and plexiform layers. (F1–F3) Pard3 staining (red) was seen at the OLM (arrowhead), but did not colocalize with acetylated tubulin signal in the cilia (arrows). All sections were counterstained with DAPI (blue). Scale bar = 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ift88 Ab1-ift88 AB/EKW standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
pard3ab Ab3-pard3 AB/EKW standard conditions Day 5 Not Detected eye photoreceptor cell photoreceptor connecting cilium IHC
Ab3-pard3 Day 5 outer limiting membrane IHC
Ab3-pard3 Day 5 retinal outer plexiform layer IHC
Ab3-pard3 Day 5 retinal photoreceptor layer cell junction IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-prkcz IHC AB/EKW standard conditions Day 5 Not Detected eye photoreceptor cell photoreceptor connecting cilium
IHC Day 5 outer limiting membrane
IHC Day 5 retinal photoreceptor layer apical region
IHC Day 5 retinal photoreceptor layer cell junction
Ab1-tjp1 IHC AB/EKW standard conditions Day 5 outer limiting membrane
Ab1-tuba IHC AB/EKW standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One