FIGURE

Fig. 11

ID

ZDB-FIG-150615-11

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

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Fig. 11

Pharmacological inhibition of PrkC activity reduces cilia length and causes opsin mislocalization. (A–C) Transverse cryosections of 4 dpf retinas were stained with phalloidin to label actin (red) and 1D1 to label rhodopsin (green). (D–F) Immunohistochemistry on cryosections to label Ift88 in cilia (red) and acetylated tubulin to label ciliary microtubules (green). Arrows identify cilia. Sections were also counterstained with DAPI (blue). (G) Quantification of cilia length. Cilia length in 4 dpf wild-type photoreceptors (43.2 microns) and the non-myristoylated control inhibitor (48.9 µm) were not statistically different from each other, but both were statistically different from larvae treated with the PrkC inhibitor (27.1 microns). Error bars show standard deviation. (***p≤0.0001) Scale bar = 10 μm.

Expression Data

Genes:	ift88 rho
Antibodies:	Ab1-ift88 Ab1-rho Ab1-tuba
Fish:	AB/EKW
Condition:	chemical treatment: EC 2.7.11.13 (protein kinase C) inhibitor
Anatomical Terms:	eye photoreceptor cell photoreceptor connecting cilium eye photoreceptor cell photoreceptor inner segment eye photoreceptor cell photoreceptor outer segment
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/EKW
Condition:	chemical treatment: EC 2.7.11.13 (protein kinase C) inhibitor
Observed In:	eye photoreceptor cell apical region photoreceptor cell photoreceptor connecting cilium
Stage:	Day 4

Phenotype Detail

Acknowledgments

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