ZFIN ID: ZDB-FIG-150615-11
Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors. PLoS One   9:e104661 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 11 Pharmacological inhibition of PrkC activity reduces cilia length and causes opsin mislocalization. (A–C) Transverse cryosections of 4 dpf retinas were stained with phalloidin to label actin (red) and 1D1 to label rhodopsin (green). (D–F) Immunohistochemistry on cryosections to label Ift88 in cilia (red) and acetylated tubulin to label ciliary microtubules (green). Arrows identify cilia. Sections were also counterstained with DAPI (blue). (G) Quantification of cilia length. Cilia length in 4 dpf wild-type photoreceptors (43.2 microns) and the non-myristoylated control inhibitor (48.9 µm) were not statistically different from each other, but both were statistically different from larvae treated with the PrkC inhibitor (27.1 microns). Error bars show standard deviation. (***p≤0.0001) Scale bar = 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ift88 Ab1-ift88 AB/EKW control Day 4 eye photoreceptor cell photoreceptor connecting cilium IHC
Ab1-ift88 AB/EKW standard conditions Day 4 eye photoreceptor cell photoreceptor connecting cilium IHC
Ab1-ift88 AB/EKW chemical treatment: EC 2.7.11.13 (protein kinase C) inhibitor Day 4 eye photoreceptor cell photoreceptor connecting cilium IHC
rho Ab1-rho AB/EKW control Day 4 eye photoreceptor cell photoreceptor outer segment IHC
Ab1-rho AB/EKW standard conditions Day 4 eye photoreceptor cell photoreceptor outer segment IHC
Ab1-rho AB/EKW chemical treatment: EC 2.7.11.13 (protein kinase C) inhibitor Day 4 eye photoreceptor cell photoreceptor inner segment IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC AB/EKW control Day 4 eye photoreceptor cell photoreceptor connecting cilium
IHC AB/EKW standard conditions Day 4 eye photoreceptor cell photoreceptor connecting cilium
IHC AB/EKW chemical treatment: EC 2.7.11.13 (protein kinase C) inhibitor Day 4 eye photoreceptor cell photoreceptor connecting cilium
Phenotype Details
Fish Conditions Stage Phenotype
AB/EKW chemical treatment: EC 2.7.11.13 (protein kinase C) inhibitor Day 4 eye photoreceptor cell apical region decreased length, abnormal
Day 4 photoreceptor cell photoreceptor connecting cilium decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One