FIGURE

Fig. 3

ID

ZDB-FIG-150615-3

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

Other Figures

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Fig. 3

Effects of pard3 suppression on apical determinants. Immunohistochemistry was performed on transverse cryosections of wild-type and pard3 morphants. Phalloidin was used to label actin (red) in all panels. (A–B) Transverse cryosections of wild-type and pard3 morphants stained with phalloidin (red) and PrkCi immunoreactivity (green). Arrows mark longitudinal fibers in the ellipsoid. (C–D) Cryosections stained with phalloidin (red) and Crb immunoreactivity (green). (E–F) Cryosections stained with phalloidin (red) and ZO-1 immunoreactivity (green). Sections were also counterstained with DAPI (blue). ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer. Scale bar = 10 µm.

Expression Data

Antibodies:	Ab1-crb Ab1-prkcz Ab1-tjp1
Fish:	AB/EKW AB/EKW + MO1-pard3ab
Knockdown Reagent:	MO1-pard3ab
Anatomical Terms:	eye photoreceptor cell apical region outer limiting membrane
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/EKW + MO1-pard3ab
Knockdown Reagent:	MO1-pard3ab
Observed In:	eye photoreceptor cell apical region
Stage:	Day 5

Phenotype Detail

Acknowledgments

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