ZFIN ID: ZDB-FIG-150511-13
Otis et al., 2015 - Zebrafish as a model for apolipoprotein biology: Comprehensive expression analysis and a role for ApoA-IV in regulating food intake. Disease models & mechanisms   8(3):295-309 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 6

Fig. 6

High-fat feed increases expression of apolipoproteins in larval gut. (A) Representative images of mRNA expression as observed by ISH of the zebrafish apoA-I, apoB, apoE and apoA-IV genes in 6-dpf nacre-/- larvae. Larvae have either never eaten exogenous food (Unfed), or have been fed a high-fat, 5% chicken egg yolk meal for 4 hours (Fed). ISH was performed in triplicate with n≥5 larvae; no staining was observed in sense probed (supplementary material Figs S1-S4). (B) A high-fat meal increases zebrafish apoA-IVa, apoA-IVb.2, and apoA-IVb.3 mRNA expression in the gut (intestine, liver, pancreas). Real-time PCR quantification of apolipoprotein transcriptional response in the 6-dpf zebrafish gut to 1, 2, 3 or 4 hours of a 10% chicken egg yolk feed (n=3; 10 pooled larval guts per experiment). Groups with the same letter are not significantly different (one-way ANOVA, P<0.05, n=3).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
apoa1a mitfaw2/w2 control Day 6 intestine ISH
apoa1b mitfaw2/w2 control Day 6 intestine ISH
Day 6 liver ISH
apoa4a mitfaw2/w2 control Day 6 intestine ISH
apoa4b.1 mitfaw2/w2 control Day 6 intestine ISH
Day 6 liver ISH
apoa4b.2 mitfaw2/w2 control Day 6 intestine ISH
apoa4b.3 mitfaw2/w2 control Day 6 intestine ISH
apoba mitfaw2/w2 control Day 6 intestine ISH
Day 6 liver ISH
apobb.1 mitfaw2/w2 control Day 6 intestine ISH
Day 6 liver ISH
apobb.2 mitfaw2/w2 control Day 6 Not Detected intestine ISH
Day 6 Not Detected liver ISH
apoea mitfaw2/w2 control Day 6 intestine ISH
apoeb mitfaw2/w2 control Day 6 intestine ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.