ZFIN ID: ZDB-FIG-150511-13
Otis et al., 2015 - Zebrafish as a model for apolipoprotein biology: Comprehensive expression analysis and a role for ApoA-IV in regulating food intake. Disease models & mechanisms   8(3):295-309 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 6

Fig. 6

High-fat feed increases expression of apolipoproteins in larval gut. (A) Representative images of mRNA expression as observed by ISH of the zebrafish apoA-I, apoB, apoE and apoA-IV genes in 6-dpf nacre-/- larvae. Larvae have either never eaten exogenous food (Unfed), or have been fed a high-fat, 5% chicken egg yolk meal for 4 hours (Fed). ISH was performed in triplicate with n≥5 larvae; no staining was observed in sense probed (supplementary material Figs S1-S4). (B) A high-fat meal increases zebrafish apoA-IVa, apoA-IVb.2, and apoA-IVb.3 mRNA expression in the gut (intestine, liver, pancreas). Real-time PCR quantification of apolipoprotein transcriptional response in the 6-dpf zebrafish gut to 1, 2, 3 or 4 hours of a 10% chicken egg yolk feed (n=3; 10 pooled larval guts per experiment). Groups with the same letter are not significantly different (one-way ANOVA, P<0.05, n=3).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
apoa1a mitfaw2/w2 control Day 6 intestine ISH
apoa1b mitfaw2/w2 control Day 6 intestine ISH
Day 6 liver ISH
apoa4a mitfaw2/w2 control Day 6 intestine ISH
apoa4b.1 mitfaw2/w2 control Day 6 intestine ISH
Day 6 liver ISH
apoa4b.2 mitfaw2/w2 control Day 6 intestine ISH
apoa4b.3 mitfaw2/w2 control Day 6 intestine ISH
apoba mitfaw2/w2 control Day 6 intestine ISH
Day 6 liver ISH
apobb.1 mitfaw2/w2 control Day 6 intestine ISH
Day 6 liver ISH
apobb.2 mitfaw2/w2 control Day 6 Not Detected intestine ISH
Day 6 Not Detected liver ISH
apoea mitfaw2/w2 control Day 6 intestine ISH
apoeb mitfaw2/w2 control Day 6 intestine ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.