ZFIN ID: ZDB-FIG-150511-9
Otis et al., 2015 - Zebrafish as a model for apolipoprotein biology: Comprehensive expression analysis and a role for ApoA-IV in regulating food intake. Disease models & mechanisms   8(3):295-309 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 75%-epiboly to Day 6

Fig. 2

Developmental mRNA expression patterns of apoA-Ia and apoA-Ib. In situ hybridization of apoA-Ia and apoA-Ib during gastrulation [80-100% epiboly (E)], somitogenesis [15-20 somite (S)], and daily until 6 dpf. Both genes localize to the YSL from somitogenesis through 5 dpf, apoA-Ia localizes to the intestine (I) at 6 dpf, and apoA-Ib localizes strongly to the liver (L) and weakly to the intestine at 6 dpf. All zebrafish are wild type except 6-dpf larvae, which are nacre-/-. Larvae studied at 2-5 dpf were treated with PTU to prevent pigment formation. No signal was observed for either gene at the eight-cell stage or blastulation (supplementary material Fig. S1). Experiments were performed three times for each gene at each stage with ne5 embryos or larvae per probe per experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apoa1a AB control 75%-epiboly to Bud yolk syncytial layer ISH
14-19 somites to 20-25 somites yolk syncytial layer ISH
Prim-5 yolk syncytial layer ISH
Long-pec yolk syncytial layer ISH
Protruding-mouth yolk syncytial layer ISH
Day 4 yolk syncytial layer ISH
Day 5 yolk syncytial layer ISH
mitfaw2/w2 standard conditions Day 6 intestine ISH
apoa1b AB control 75%-epiboly to Bud yolk syncytial layer ISH
14-19 somites to 20-25 somites yolk syncytial layer ISH
Prim-5 yolk syncytial layer ISH
Long-pec yolk syncytial layer ISH
Protruding-mouth yolk syncytial layer ISH
Day 4 yolk syncytial layer ISH
Day 5 yolk syncytial layer ISH
mitfaw2/w2 standard conditions Day 6 intestine ISH
Day 6 liver ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.