ZFIN ID: ZDB-FIG-150415-18
Takamiya et al., 2015 - Molecular Description of Eye Defects in the Zebrafish Pax6b Mutant, sunrise, Reveals a Pax6b-Dependent Genetic Network in the Developing Anterior Chamber. PLoS One   10:e0117645 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 2

In situ mRNA expression analysis in the adult zebrafish cornea.

Two major classes of expression patterns are shown for selected genes: genes mainly expressed in the corneal endothelium (paired-like homeodomain 2 (A-A′) and catenin, beta 2 (B)) and mainly in the corneal epithelium (frizzled-related protein (C), ets variant 4 (previously known as pea3, D-D′), paired box 6b (E), forkhead box C1a (F), tenascin W (G), GDP-mannose 4,6-dehydratase (H), rho-associated, coiled-coil containing protein kinase 2a (I), zgc:85975 (J), zgc:92061 (K), zgc:92380 (L), scinderin like b (M) and retinal pigment epithelium-specific protein 65a (N-N′′). A control without probe is shown in the panel O. Filled arrows, open arrows, arrowheads and asterisks indicate expression in the corneal epithelial, stromal, endothelial layers and the annular ligament, respectively. The black “flakes” (indicated by p in the panels of A, B, K and N′′) are derivatives of pigmentation unavoidably introduced during cryosectioning and its post-processing. Scale bar: 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ckap4 WT standard conditions Adult corneal epithelium ISH
ctnnb2 WT standard conditions Adult corneal endothelium ISH
etv4 WT standard conditions Adult corneal epithelium ISH
foxc1a WT standard conditions Adult corneal epithelium ISH
Adult corneal stroma ISH
frzb WT standard conditions Adult corneal epithelium ISH
gmds WT standard conditions Adult corneal epithelium ISH
krt97 WT standard conditions Adult corneal epithelium ISH
pax6b WT standard conditions Adult corneal epithelium ISH
pitx2 WT standard conditions Adult corneal endothelium ISH
Adult corneal epithelium ISH
Adult corneal stroma ISH
rock2a WT standard conditions Adult corneal epithelium ISH
rpe65a WT standard conditions Adult annular ligament ISH
Adult corneal epithelium ISH
scinlb WT standard conditions Adult corneal epithelium ISH
tnn WT standard conditions Adult corneal epithelium ISH
zgc:92380 WT standard conditions Adult corneal epithelium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One