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ZFIN ID: ZDB-FIG-150415-22
Takamiya et al., 2015 - Molecular Description of Eye Defects in the Zebrafish Pax6b Mutant, sunrise, Reveals a Pax6b-Dependent Genetic Network in the Developing Anterior Chamber. PLoS One   10:e0117645 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Days 7-13

Fig. 7

Gene expression in the cornea is severely affected in pax6b mutants.

The distribution of the mRNA of cornea genes was compared at 7 dpf between wildtype (A-R) and pax6b mutant embryos (A′-R′). Each panel represents a transverse section through the eye and the lens. The gene transcripts analysed by in situ hybridization are zgc:63489 (A-A′), sox3 (B-B′), tfap2a (C-C′), foxc1a (D-D′), anxa1b (E-E′), fgd (F-F′), zgc:92380 (G-G′), jup (H-H′), mpzl3 (I-I′), pitx2 (J-J′), ctnnb2 (K-K′), dcn (L-L′), fabp7a (M-M′), lima1 (N-N′), rrbp1 (O-O′), serpinb1l1 (P-P′), zgc:73226 (Q-Q′) and olfml3b (R-R′). (A-I′) Examples for the loss of gene expression in the pax6b mutant (asterisks). (J-R′) Genes whose expression is ectopically induced in the pax6b mutant. The insets (j′-r′) represent magnified views of corresponding cornea regions (rectangular areas in J′-R′); arrowheads, filled arrows and open arrows indicate the corneal epithelium, the endothelium and the lens epithelium. The ectopic expression in pax6b mutants was mostly observed in the endothelial layer or in the iridocorneal angle. In contrast, the loss of gene expression in the pax6b mutant was mostly detected in the epithelial layer (asterisks in A-I). Scale bar: 64 µm (A-R′) and 20 µm (j′-r′).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
anxa1b WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
ctnnb2 WT standard conditions Days 7-13 corneal endothelium ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
Days 7-13 corneal epithelium ISH
Days 7-13 lens epithelium ISH
dcn WT standard conditions Days 7-13 Not Detected cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
fabp7a WT standard conditions Days 7-13 Not Detected cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
Days 7-13 lens epithelium ISH
fgd WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
foxc1a WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
jupa WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
lima1a WT standard conditions Days 7-13 Not Detected cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
mpzl3 WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
olfml3b WT standard conditions Days 7-13 Not Detected cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
pitx2 WT standard conditions Days 7-13 corneal epithelium ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
Days 7-13 Not Detected corneal epithelium ISH
rrbp1b WT standard conditions Days 7-13 Not Detected cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
Days 7-13 corneal epithelium ISH
Days 7-13 lens epithelium ISH
serpinb1l1 WT standard conditions Days 7-13 Not Detected cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
sox3 WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
tfap2a WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
zbtb7b WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
zgc:73226 WT standard conditions Days 7-13 Not Detected cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 corneal endothelium ISH
zgc:92380 WT standard conditions Days 7-13 cornea ISH
pax6btq253a/tq253a standard conditions Days 7-13 Not Detected cornea ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One