ZFIN ID: ZDB-FIG-150415-20
Takamiya et al., 2015 - Molecular Description of Eye Defects in the Zebrafish Pax6b Mutant, sunrise, Reveals a Pax6b-Dependent Genetic Network in the Developing Anterior Chamber. PLoS One   10:e0117645 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Days 7-13 to Days 30-44

Fig. 4

Comparison of gene expression in the cornea of wild type zebrafish at 7 dpf (A-O) and 1-month stage (A′-O′).

Transverse sections with dorsal up. (A) Negative controls without probe showed no staining at both stages, except for background staining in the lens (ln, outlined by a stippled line). p: pigmentation in the iris and the retinal pigment epithelium. (B-K′) Examples of genes expressed at both 7 dpf and 1-month stage are shown. A subset of genes (B-F′) is mainly expressed in the corneal epithelium (arrowheads) and another subset (G-K′) shows expression in the corneal endothelium (arrows). (L-O′) Examples of genes that are mainly expressed at the 1-month stage. A subset of genes (M-O′) is expressed in the corneal epithelium (arrowheads) and another subset (L-L′) in the corneal endothelium (arrows). The gene symbols are indicated in the bar above each pair of sections. Scale bar: (A-O) 80 µm; (A′-O′) 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dbnla WT standard conditions Days 7-13 corneal epithelium ISH
Days 30-44 corneal epithelium ISH
frzb WT standard conditions Days 7-13 corneal endothelium ISH
Days 30-44 corneal endothelium ISH
gmds WT standard conditions Days 7-13 Not Detected cornea ISH
Days 30-44 corneal epithelium ISH
jupa WT standard conditions Days 7-13 corneal epithelium ISH
Days 30-44 corneal endothelium ISH
Days 30-44 corneal epithelium ISH
krt97 WT standard conditions Days 7-13 corneal epithelium ISH
Days 30-44 corneal epithelium ISH
nudt9 WT standard conditions Days 7-13 corneal epithelium ISH
Days 30-44 corneal epithelium ISH
pax6b WT standard conditions Days 7-13 corneal endothelium ISH
Days 30-44 corneal endothelium ISH
reep3b WT standard conditions Days 7-13 corneal epithelium ISH
Days 30-44 corneal endothelium ISH
rpe65a WT standard conditions Days 7-13 corneal endothelium ISH
Days 30-44 corneal endothelium ISH
scel WT standard conditions Days 7-13 corneal epithelium ISH
Days 30-44 corneal epithelium ISH
sh3d21 WT standard conditions Days 7-13 Not Detected cornea ISH
Days 30-44 corneal epithelium ISH
zgc:73226 WT standard conditions Days 7-13 corneal endothelium ISH
Days 30-44 corneal endothelium ISH
zgc:92380 WT standard conditions Days 7-13 corneal epithelium ISH
Days 30-44 corneal epithelium ISH
zgc:111983 WT standard conditions Days 7-13 Not Detected cornea ISH
Days 30-44 corneal epithelium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One