ZFIN ID: ZDB-FIG-150313-34
Huc-Brandt et al., 2014 - Zebrafish Prion Protein PrP2 Controls Collective Migration Process during Lateral Line Sensory System Development. PLoS One   9:e113331 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Day 5 to Days 7-13
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 8

Loss of PLL nerve fasciculation and associated myelination in absence of PrP2.

A–C. In control larvae at 5 dpf, sox10-GFP cells are tightly organized along the PLL nerve (nbt-dsred). D–F. Double morphant PrP2/p53 displayed an enlarged PLL nerve (with defasciculated axons, arrows) and rounded Schwann cells (arrowheads). G–G′. In 5 dpf larvae MBP labeling is observed in close apposition to sox10-GFP cells and formed a homogeneous line. H–H′. MBP labeling is altered and partially missing while Schwann cells are disorganized. I–I′. At 7 dpf, control larvae display tightly and regularly organized sox10-GFP positive cells. J–K′. In PrP2/p53 morphants (J–J′′), loosened Schwann cell processes are observed (arrows) as well as rounded cells (arrowheads) in PrP2 morphants (K–K′). Results obtained from five independent experiments (n = 160 embryos).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed ba4Tg; zf148Tg control Day 5 posterior lateral line axon IHC
ba4Tg; zf148Tg + MO4-tp53 + MO5-prnprs3 standard conditions Day 5 posterior lateral line axon IHC
GFP ba4Tg control Day 5 posterior lateral line myelinating Schwann cell IHC
Days 7-13 posterior lateral line myelinating Schwann cell IHC
ba4Tg + MO4-tp53 + MO5-prnprs3 standard conditions Day 5 posterior lateral line myelinating Schwann cell IHC
Days 7-13 posterior lateral line myelinating Schwann cell IHC
ba4Tg + MO5-prnprs3 standard conditions Days 7-13 posterior lateral line myelinating Schwann cell IHC
ba4Tg; zf148Tg control Day 5 posterior lateral line myelinating Schwann cell IHC
ba4Tg; zf148Tg + MO4-tp53 + MO5-prnprs3 standard conditions Day 5 posterior lateral line myelinating Schwann cell IHC
mbpa ba4Tg control Day 5 posterior lateral line myelinating Schwann cell IHC
ba4Tg + MO4-tp53 + MO5-prnprs3 standard conditions Day 5 posterior lateral line myelinating Schwann cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ba4Tg + MO4-tp53 + MO5-prnprs3 standard conditions Day 5 myelination of posterior lateral line nerve axons decreased occurrence, abnormal
Day 5 posterior lateral line axon defasciculated, abnormal
Day 5 posterior lateral line development decreased process quality, abnormal
Day 5 posterior lateral line increased diameter, abnormal
Day 5 posterior lateral line primordium circular, abnormal
Days 7-13 myelination of posterior lateral line nerve axons decreased occurrence, abnormal
Days 7-13 posterior lateral line axon defasciculated, abnormal
Days 7-13 posterior lateral line development decreased process quality, abnormal
Days 7-13 posterior lateral line increased diameter, abnormal
Days 7-13 posterior lateral line primordium circular, abnormal
ba4Tg + MO5-prnprs3 standard conditions Day 5 myelination of posterior lateral line nerve axons decreased occurrence, abnormal
Day 5 posterior lateral line axon defasciculated, abnormal
Day 5 posterior lateral line development decreased process quality, abnormal
Day 5 posterior lateral line increased diameter, abnormal
Day 5 posterior lateral line primordium circular, abnormal
Days 7-13 myelination of posterior lateral line nerve axons decreased occurrence, abnormal
Days 7-13 posterior lateral line axon defasciculated, abnormal
Days 7-13 posterior lateral line development decreased process quality, abnormal
Days 7-13 posterior lateral line increased diameter, abnormal
Days 7-13 posterior lateral line primordium circular, abnormal
ba4Tg; zf148Tg + MO4-tp53 + MO5-prnprs3 standard conditions Day 5 myelination of posterior lateral line nerve axons decreased occurrence, abnormal
Day 5 posterior lateral line axon defasciculated, abnormal
Day 5 posterior lateral line development decreased process quality, abnormal
Day 5 posterior lateral line increased diameter, abnormal
Day 5 posterior lateral line myelinating Schwann cell circular, abnormal
ba4Tg; zf148Tg + MO5-prnprs3 standard conditions Day 5 myelination of posterior lateral line nerve axons decreased occurrence, abnormal
Day 5 posterior lateral line axon defasciculated, abnormal
Day 5 posterior lateral line development decreased process quality, abnormal
Day 5 posterior lateral line increased diameter, abnormal
Day 5 posterior lateral line myelinating Schwann cell circular, abnormal
Acknowledgments:
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