ZFIN ID: ZDB-FIG-150313-27
Huc-Brandt et al., 2014 - Zebrafish Prion Protein PrP2 Controls Collective Migration Process during Lateral Line Sensory System Development. PLoS One   9:e113331 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 1

PrP2 decrease expression results in abnormal PLL development.

A. In control embryos at 48 hours post fertilization (hpf) from nbt-dsred line that labels neurons and axons, the PLL nerve develops from the PLL ganglia until the tip of the tail (arrow). B–C. In PrP2-MO embryos, a range of defects for the PLL nerve is observed with premature arrest (B, C). D. Control PLL nerve fibers are tightly while PrP2-MO fibers exhibit abnormal branches were observed in severe cases (E). F. In control claudinB-GFP embryos at 48 hpf all derivatives issuing from the primordium express GFP and appear normal. Five regularly spaced neuromasts are present, with 3 terminal neuromasts at the tip of the tail. Due to embryo transparency, the neuromasts on the other side of the embryo are also visible. In PrP2-MO (G) and PrP2-MO/p53-MO (H) injected embryos (in order to avoid off-target defects), neuromast numbers are reduced, and often irregularly spaced. I–J. Western blot analysis of PrP2 expression using SAF84 antibody shows a decrease of the PrP2 protein after morpholino injections (n = 3 independent experiments).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed zf148Tg control Long-pec posterior lateral line axon IFL
Long-pec posterior lateral line ganglion IFL
zf148Tg + MO4-tp53 + MO5-prnprs3 standard conditions Long-pec posterior lateral line axon IFL
Long-pec posterior lateral line ganglion IFL
zf148Tg + MO5-prnprs3 standard conditions Long-pec posterior lateral line axon IFL
Long-pec posterior lateral line ganglion IFL
EGFP zf106Tg control Long-pec posterior lateral line neuromast IFL
zf106Tg + MO4-tp53 + MO5-prnprs3 standard conditions Long-pec posterior lateral line neuromast IFL
zf106Tg + MO5-prnprs3 standard conditions Long-pec posterior lateral line neuromast IFL
prnprs3 Ab2-prnp WT control Long-pec whole organism WB
Ab2-prnp WT + MO4-tp53 + MO5-prnprs3 standard conditions Long-pec whole organism WB
Ab2-prnp WT + MO5-prnprs3 standard conditions Long-pec whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf148Tg + MO4-tp53 + MO5-prnprs3 standard conditions Long-pec posterior lateral line decreased length, abnormal
Long-pec posterior lateral line development decreased process quality, abnormal
Long-pec posterior lateral line ganglion size, normal
Long-pec posterior lateral line has fewer parts of type posterior lateral line neuromast, abnormal
zf148Tg + MO5-prnprs3 standard conditions Long-pec posterior lateral line decreased length, abnormal
Long-pec posterior lateral line development decreased process quality, abnormal
Long-pec posterior lateral line ganglion size, normal
Long-pec posterior lateral line has fewer parts of type posterior lateral line neuromast, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One