ZFIN ID: ZDB-FIG-150313-32
Huc-Brandt et al., 2014 - Zebrafish Prion Protein PrP2 Controls Collective Migration Process during Lateral Line Sensory System Development. PLoS One   9:e113331 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 6

Delocalisation of E-cadherin and beta-catenin in primordium cells in absence of PrP2.

A. Schematic representation of claudinB-GFP embryo at 30 hpf, primordium structure, and transversal view of a rosette showing the level of confocal focus plan. B–B′′. In control embryos at 30 hpf, E-cadherin is expressed at the membrane level at the basal side of the primordium. C–C′′. In morphant embryos, E-cadherin is observed in the cytoplasm and cellular membrane at basal and apical levels. The staining pattern is more punctuated in morphant compared to control. D, E. In control embryos, beta catenin expression is observed at the membrane level and at the center of rosette structures. F, G. In morphant, small rounded primordium shows a membrane homogeneous pattern with no rosette.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf106Tg control Prim-15 posterior lateral line primordium IHC
zf106Tg + MO5-prnprs3 standard conditions Prim-15 posterior lateral line primordium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf106Tg + MO5-prnprs3 standard conditions Prim-15 posterior lateral line primordium adherens junction decreased object quality, abnormal
Prim-15 posterior lateral line primordium adherens junction maintenance decreased process quality, abnormal
Acknowledgments:
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