|
Fig. 8
Loss of PLL nerve fasciculation and associated myelination in absence of PrP2.
A?C. In control larvae at 5 dpf, sox10-GFP cells are tightly organized along the PLL nerve (nbt-dsred). D?F. Double morphant PrP2/p53 displayed an enlarged PLL nerve (with defasciculated axons, arrows) and rounded Schwann cells (arrowheads). G?G′. In 5 dpf larvae MBP labeling is observed in close apposition to sox10-GFP cells and formed a homogeneous line. H?H′. MBP labeling is altered and partially missing while Schwann cells are disorganized. I?I′. At 7 dpf, control larvae display tightly and regularly organized sox10-GFP positive cells. J?K′. In PrP2/p53 morphants (J?J′′), loosened Schwann cell processes are observed (arrows) as well as rounded cells (arrowheads) in PrP2 morphants (K?K′). Results obtained from five independent experiments (n = 160 embryos).