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ZFIN ID: ZDB-FIG-140915-19
Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development. PLoS One   9:e99210 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 9

Knockdown of ambra1 interferes with myosin organization in slow and fast muscles and with myosepta.

Lateral views of 3 dpf embryos labeled with the F59 antibody (slow muscle fibers) and with the F310 antibody (fast muscle fibers), showing abnormally organized myofilaments in ATG-morphant embryos when compared to WT and 5 m-control embryos. Slow fibers are thinner in ATG-morphant embryos, whereas fast fibers of splice-morphant embryos display a wavy phenotype, visibly different from controls. The asterisks indicate broken or missing muscle fibers. Laminin labeling highlights the loss of the V-shape arrangement of somites and reveals interrupted myosepta (arrowheads) in ATG-morphant embryos. Defects in myosin organization and myosepta structure are rescued with the by co-injection with human AMBRA1 mRNA. Anterior is to the left and dorsal up.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ambra1a standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth myoseptum morphology, abnormal
Protruding-mouth myosin filament organization disrupted, abnormal
Protruding-mouth skeletal myofibril assembly disrupted, abnormal
Protruding-mouth somite shape, abnormal
AB + MO1-ambra1a + MO1-ambra1b standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth myoseptum morphology, abnormal
Protruding-mouth myosin filament organization disrupted, abnormal
Protruding-mouth skeletal myofibril assembly disrupted, abnormal
Protruding-mouth somite shape, abnormal
AB + MO1-ambra1b standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth myoseptum morphology, abnormal
Protruding-mouth myosin filament organization disrupted, abnormal
Protruding-mouth skeletal myofibril assembly disrupted, abnormal
Protruding-mouth somite shape, abnormal
AB + MO2-ambra1a standard conditions Protruding-mouth fast muscle cell undulate, abnormal
AB + MO2-ambra1b standard conditions Protruding-mouth fast muscle cell undulate, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One