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ZFIN ID: ZDB-FIG-140915-14
Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development. PLoS One   9:e99210 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 4

Abnormal morphology of ambra1 knockdown embryos, as revealed by toluidine blue staining.

Representative longitudinal and cross sections of 3 dpf control and ambra1 morphant embryos. Muscles of ambra1 ATG-morphants show a severe phenotype, with misaligned myofibers scattered in the somitic compartment. Black arrows, areas devoid of staining; white arrows, large myonuclei with condensed chromatin; black arrowheads, interruption of myoseptum; asterisks, opaque material replacing lost myofibers.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ambra1a standard conditions Protruding-mouth muscle cell nucleus morphology, abnormal
Protruding-mouth myoseptum unstructured, abnormal
Protruding-mouth trunk musculature muscle cell disorganized, abnormal
AB + MO1-ambra1a + MO1-ambra1b standard conditions Protruding-mouth muscle cell nucleus morphology, abnormal
Protruding-mouth myoseptum unstructured, abnormal
Protruding-mouth trunk musculature muscle cell disorganized, abnormal
AB + MO1-ambra1b standard conditions Protruding-mouth muscle cell nucleus morphology, abnormal
Protruding-mouth myoseptum unstructured, abnormal
Protruding-mouth trunk musculature muscle cell disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One