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ZFIN ID: ZDB-FIG-140915-13
Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development. PLoS One   9:e99210 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3

Abnormal morphology of ambra1 knockdown embryos, as revealed by haematoxylin/eosin staining.

Representative longitudinal sections of 3 dpf control and ambra1 morphant embryos. Myofibers of ATG- and co-injected morphants muscles are highly disorganized and display irregular myosepta boundaries. The phenotype of splice-morphants is much less severe when compared to WT and 5 m-control embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ambra1a standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth muscle cell nucleus increased amount, abnormal
Protruding-mouth myoseptum morphology, abnormal
AB + MO1-ambra1a + MO1-ambra1b standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth muscle cell nucleus increased amount, abnormal
Protruding-mouth myoseptum morphology, abnormal
AB + MO1-ambra1b standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth muscle cell nucleus increased amount, abnormal
Protruding-mouth myoseptum morphology, abnormal
AB + MO2-ambra1a standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth myoseptum decreased thickness, abnormal
AB + MO2-ambra1b standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth myoseptum decreased thickness, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One