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ZFIN ID: ZDB-FIG-140915-18
Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development. PLoS One   9:e99210 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 8

Ultrastructural analysis of ambra1 morphants muscles reveals disorganized subcellular structures.

When compared to WT and 5-control (not shown) embryos, ambra1 morphant embryos display alterations of mitochondria (row A), nuclei (row B) and triads (row C), perturbation of the hexagonal arrangement of thick and thin filaments (row D), and dilations of the endoplasmic reticulum (row E). Muscles of morphant embryos show the presence of areas with reduced thin filaments (black circles in row D) adjacent to more normal-appearing hexagonal structures (white circles in row D). Co-injected double morphant embryos show exacerbated defects of these structures, whereas defects are barely evident in splice-morphants.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ambra1a standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth muscle cell nucleus morphology, abnormal
Protruding-mouth muscle mitochondrion morphology, abnormal
Protruding-mouth muscle sarcoplasmic reticulum dilated, abnormal
Protruding-mouth muscle striated muscle thin filament separated from muscle myosin filament, abnormal
AB + MO1-ambra1a + MO1-ambra1b standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth muscle cell nucleus morphology, abnormal
Protruding-mouth muscle mitochondrion morphology, abnormal
Protruding-mouth muscle sarcoplasmic reticulum dilated, abnormal
Protruding-mouth muscle striated muscle thin filament separated from muscle myosin filament, abnormal
AB + MO1-ambra1b standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth muscle cell nucleus morphology, abnormal
Protruding-mouth muscle mitochondrion morphology, abnormal
Protruding-mouth muscle sarcoplasmic reticulum dilated, abnormal
Protruding-mouth muscle striated muscle thin filament separated from muscle myosin filament, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One