ZFIN ID: ZDB-FIG-140915-12
Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development. PLoS One   9:e99210 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Fig. 2

In situ hybridization analysis of myoD1 expression in ambra1 knockdown embryos.

Expression of myoD1, analyzed in embryos injected with the indicated MOs at 10 and 20 hpf, is affected in ambra1 ATG-morphants and in co-injected morphants. No differences are evident in ambra1 splice-morphants when compared to WT and 5 m-control embryos. Embryos are shown by dorsal view, anterior side on the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 AB control Bud adaxial cell ISH
20-25 somites somite ISH
AB + MO1-ambra1a standard conditions Bud adaxial cell ISH
20-25 somites somite ISH
AB + MO1-ambra1a + MO1-ambra1b standard conditions Bud adaxial cell ISH
20-25 somites somite ISH
AB + MO1-ambra1b standard conditions Bud adaxial cell ISH
20-25 somites somite ISH
AB + MO2-ambra1a standard conditions Bud adaxial cell ISH
20-25 somites somite ISH
AB + MO2-ambra1b standard conditions Bud adaxial cell ISH
20-25 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ambra1a standard conditions 20-25 somites notochord undulate, abnormal
20-25 somites somite morphology, abnormal
20-25 somites whole organism anterior-posterior axis shortened, abnormal
AB + MO1-ambra1a + MO1-ambra1b standard conditions 20-25 somites notochord undulate, abnormal
20-25 somites somite morphology, abnormal
20-25 somites whole organism anterior-posterior axis shortened, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One