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ZFIN ID: ZDB-FIG-140915-11
Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development. PLoS One   9:e99210 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 1

Ablation of ambra1 results in reduced birefringence in zebrafish embryos.

(A). Representative images under normal and polarized light of 3-dpf live embryos injected with the indicated MOs. ATG-morphant embryos show reduced size, curved shape, pericardial edema and reduced birefringence when compared to WT and 5 m-control embryos. No visible abnormalities are evident in splice-morphants. The phenotypic defects of ATG-morphant embryos, including birefringence, are rescued by co-injection with 10 ng/embryo of human AMBRA1 mRNA. (B). Quantification of embryo trunk muscles birefringence shows a severe and statistically significant reduction in ambra1 ATG- and in co-injected morphants. The birefringence is faintly reduced in ambra1 splice-morphants, whereas WT and 5 m-control embryos display highly birefringent skeletal muscles. Muscle birefringence is statistically increased when ATG- and co-injected morphants are co-injected with human AMBRA1 mRNA (***, P<0.001).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ambra1a standard conditions Protruding-mouth embryo development disrupted, abnormal
Protruding-mouth post-vent region curved, abnormal
Protruding-mouth whole organism decreased size, abnormal
Protruding-mouth whole organism refractivity, abnormal
AB + MO1-ambra1b standard conditions Protruding-mouth embryo development disrupted, abnormal
Protruding-mouth post-vent region curved, abnormal
Protruding-mouth whole organism decreased size, abnormal
Protruding-mouth whole organism refractivity, abnormal
AB + MO2-ambra1a standard conditions Protruding-mouth whole organism morphology, normal
AB + MO2-ambra1b standard conditions Protruding-mouth whole organism morphology, normal
WT + MO1-ambra1a + MO1-ambra1b standard conditions Protruding-mouth embryo development disrupted, abnormal
Protruding-mouth post-vent region curved, abnormal
Protruding-mouth whole organism decreased size, abnormal
Protruding-mouth whole organism refractivity, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One