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ZFIN ID: ZDB-FIG-140915-17
Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development. PLoS One   9:e99210 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 7

Ultrastructural analysis of ambra1 morphants muscles reveals disorganized sarcomeres.

Representative electron micrographs of cross and longitudinal sections of 3 dpf (WT, panels A1–A4), ambra1a ATG-morphant (panels B1-B4), ambra1a splice-morphant (panels C1–C4), ambra1b ATG-morphant (panels D1–D4), ambra1b splice-morphant (panels E1-E4), and co-injected morphant (panels F1–F4) zebrafish embryos. Columns 2 and 4 show higher magnification views of the boxed areas in column 1 and 3, respectively. Muscles of WT and 5 m-control (not shown) embryos display well-organized myofibers, showing densely packed sarcomeres with regular organization of thin and thick myofilaments. ambra1 depleted muscles show a number of ultrastructural defects, with small patches of disorganized myofibers and mitochondria scattered throughout the cytoplasm. Black arrows, area with myofibrils having different orientations; white arrow, dilated sarcoplasmic reticulum not in contact with myofibrils; asterisks, fragments of torn myofibrils; M, mitochondria; N, nucleus.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ambra1a standard conditions Protruding-mouth muscle cell degenerate, abnormal
Protruding-mouth muscle disorganized, abnormal
Protruding-mouth muscle sarcomere decreased amount, abnormal
Protruding-mouth muscle sarcomere disorganized, abnormal
AB + MO1-ambra1a + MO1-ambra1b standard conditions Protruding-mouth muscle cell degenerate, abnormal
Protruding-mouth muscle disorganized, abnormal
Protruding-mouth muscle sarcomere decreased amount, abnormal
Protruding-mouth muscle sarcomere disorganized, abnormal
AB + MO1-ambra1b standard conditions Protruding-mouth muscle cell degenerate, abnormal
Protruding-mouth muscle disorganized, abnormal
Protruding-mouth muscle sarcomere decreased amount, abnormal
Protruding-mouth muscle sarcomere disorganized, abnormal
AB + MO2-ambra1a standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth muscle sarcomere disorganized, abnormal
AB + MO2-ambra1b standard conditions Protruding-mouth muscle cell disorganized, abnormal
Protruding-mouth muscle sarcomere disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One