ZFIN ID: ZDB-FIG-140317-37
Barth et al., 1999 - Bmp activity establishes a gradient of positional information throughout the entire neural plate. Development (Cambridge, England)   126(22):4977-4987
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 1-4 somites

Fig. 6 Sbn affects marginal cells fates and patterns of neurogenesis. All panels show 1-2 somite stage embryos. (A,B) Lateral views of gta2 expression (prospective epidermis) in mild (A) and severe (B) sbn- embryos. The inset panel shows expression in a wild-type embryo. Expression is retained in the anterior ventral region of the mildly affected embryo. (C,D) Lateral views of emx1 (prospective telencephalon) and fkd6 (prospective neural crest) expression in mild (C) and severe (D) sbn- embryos. The inset panel shows expression in a wild-type embryo. In the mildly affected embryo, expression is relatively normal in rostral regions, consistent with retention of gta2 expression (see A) in ventral ectoderm. However, more caudally, fkd6 expression is radialised (arrow). In the severely affected embryo, emx1 and fkd6 are expanded throughout the ventral ectoderm consistent with loss of gta2 in this region (see B). (E-G) Animal pole views of flh expression (prospective epiphysis) in progressively more severe sbn- embryos. flh expression is variably expanded in the sbn- embryos (see Fig. 2E,F for wild-type and swr- expression). (H) Dorsal and (I) lateral views of elavC expression in a sbn- embryo. Wild-type pattern of expression is shown in Fig. 3A,B. In these mildly affected sbn- embryos, interneurons are slightly expanded and dorsal sensory neurons are broadly expanded on the ventral side of the embryo. (J) Ventral view of isl1 expression in a severely affected sbn- embryo. Trigeminal and Rohon-Beard neurons are absent. (K-N) Summary schematics of patterns of neurogenesis in wild-type, sbn- and swr- embryos. In the mildly affected sbn- embryo, some non-neural ectoderm is retained and trigeminal and RB neurons are expanded on the ventral side of the embryo. In the severe sbn- embryo, trigeminal and RB neurons are lost and in the swr- embryo, interneurons are expanded throughout the ventral ectoderm. in, interneurons; me, medial neurons; mn, motor neurons; RB, Rohon-Beard neurons; V, trigeminal neurons.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
elavl3 smad5dtc24/dtc24 standard conditions 1-4 somites interneuron ISH
1-4 somites sensory neuron ISH
emx3 smad5dtc24/dtc24 standard conditions 1-4 somites presumptive telencephalon ISH
foxd3 smad5dtc24/dtc24 standard conditions 1-4 somites neural crest ISH
gata2a smad5dtc24/dtc24 standard conditions 1-4 somites Not Detected ectoderm ISH
isl1a smad5dtc24/dtc24 standard conditions 1-4 somites Not Detected Rohon-Beard neuron ISH
1-4 somites Not Detected trigeminal sensory neuron ISH
noto smad5dtc24/dtc24 standard conditions 1-4 somites neural plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
smad5dtc24/dtc24 standard conditions 1-4 somites Rohon-Beard neuron decreased amount, abnormal
1-4 somites trigeminal sensory neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.