Gene
en2a
- ID
- ZDB-GENE-980526-167
- Name
- engrailed homeobox 2a
- Symbol
- en2a Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 7 Mapping Details/Browsers
- Description
- Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within apoptotic process involved in morphogenesis; cell fate specification; and nervous system development. Predicted to be active in nucleus. Is expressed in several structures, including adaxial cell; central nervous system; muscle; neural plate; and pharyngeal arch. Human ortholog(s) of this gene implicated in autistic disorder. Orthologous to human EN2 (engrailed homeobox 2).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 106 figures from 75 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- eu759 (15 images)
Wild Type Expression Summary
- All Phenotype Data
- 1 Figure from Scholpp et al., 2001
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
No data available
| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| MO1-en2a | N/A | (5) |
| MO2-en2a | N/A | Ahmed et al., 2017 |
| MO3-en2a | N/A | Ahmed et al., 2017 |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Engrailed Homeobox Transcription Factors | Helix-turn-helix motif | Homedomain-like superfamily | Homeobox, conserved site | Homeobox engrailed, C-terminal | Homeobox engrailed-type, conserved site | Homeodomain | Homeodomain engrailed | Homeodomain, metazoa |
|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:P09015 | InterPro | 265 | |||||||||
| UniProtKB:B3DGA1 | InterPro | 265 |
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Interactions and Pathways
No data available
Plasmids
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Et(en2a-fosab:EGFP-CAAX) |
|
| 1 | (2) | |
| Tg1(en2a:EGFP) |
|
| 2 | (4) | |
| Tg2(en2a:EGFP) |
|
| 1 | (2) | |
| Tg3(en2a:EGFP) |
|
| 1 | (2) | |
| Tg4(en2a:EGFP) |
|
| 1 | (2) | |
| Tg5(en2a:EGFP) |
|
| 1 | (2) | |
| Tg6(en2a:EGFP) |
|
| 1 | (2) | |
| Tg7(en2a:EGFP) |
|
| 1 | (2) | |
| Tg8(en2a:EGFP) |
|
| 1 | (3) | |
| Tg(-8en2a:EGFP) |
|
| 1 | (2) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-105D1 | ZFIN Curated Data | |
| Contained in | BAC | CH211-150E22 | ZFIN Curated Data | |
| Encodes | EST | eu759 | Thisse et al., 2005 | |
| Encodes | cDNA | MGC:193941 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:193943 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_131044 (1) | 2901 nt | ||
| Genomic | GenBank:CU041319 (2) | 174262 nt | ||
| Polypeptide | UniProtKB:B3DGA1 (1) | 265 aa |
- Maekawa, M., Saito, S., Isobe, D., Takemoto, K., Miura, Y., Dobashi, Y., Yamasu, K. (2024) The Oct4-related PouV gene, pou5f3, mediates isthmus development in zebrafish by directly and dynamically regulating pax2a. Cells & development. 179:203933
- Altbürger, C., Holzhauser, J., Driever, W. (2023) CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain. Frontiers in Neuroanatomy. 17:11968681196868
- Baldera, D., Baxendale, S., van Hateren, N.J., Marzo, M., Glendenning, E., Geng, F.S., Yokoya, K., Knight, R.D., Whitfield, T.T. (2023) Enhancer trap lines with GFP driven by smad6b and frizzled1 regulatory sequences for the study of epithelial morphogenesis in the developing zebrafish inner ear. Journal of anatomy. 243(1):78-89
- Ulhaq, Z.S., Ogino, Y., Tse, W.K.F. (2023) FGF8 rescues motor deficits in zebrafish model of limb-girdle muscular dystrophy R18. Biochemical and Biophysical Research Communications. 652:768376-83
- Zhou, J., Yang, Y.J., Gan, R.H., Wang, Y., Li, Z., Zhang, X.J., Gui, J.F., Zhou, L. (2022) Foxl2a and Foxl2b are involved in midbrain-hindbrain boundary development in zebrafish. Gene expression patterns : GEP. 46:119286
- Zhang, H., Wang Haifang, H., Shen, X., Jia, X., Yu, S., Qiu, X., Wang, Y., Du, J., Yan, J., He, J. (2021) The landscape of regulatory genes in brain-wide neuronal phenotypes of a vertebrate brain. eLIFE. 10:
- Amblard, I., Thauvin, M., Rampon, C., Queguiner, I., Pak, V.V., Belousov, V., Prochiantz, A., Volovitch, M., Joliot, A., Vriz, S. (2020) H2O2 and Engrailed 2 paracrine activity synergize to shape the zebrafish optic tectum. Communications biology. 3:536
- Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C. (2020) Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs. Developmental Biology. 462(2):152-164
- Gonzalez-Munoz, E., Arboleda-Estudillo, Y., Chanumolu, S.K., Otu, H.H., Cibelli, J.B. (2019) Zebrafish macroH2A variants have distinct embryo localization and function. Scientific Reports. 9:8632
- Leerberg, D.M., Hopton, R.E., Draper, B.W. (2019) Fibroblast Growth Factor Receptors Function Redundantly During Zebrafish Embryonic Development. Genetics. 212(4):1301-1319
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