ZFIN ID: ZDB-FIG-130510-12
Münch et al., 2013 - Notch regulates blastema proliferation and prevents differentiation during adult zebrafish fin regeneration. Development (Cambridge, England)   140(7):1402-1411 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 5

Notch gain of function increases proliferation and is accompanied by an expansion of distal blastema marker expression into proximal regions. (A-C) BrdU-stained fin sections after a 10-hour heat-shock series (62-72 hpa) and quantification of the percentage of BrdU-labelled DAPI nuclei in the blastema: more cells incorporate BrdU in Tg(UAS:NICD) fins (B) than in wild-type fins (A). Asterisk in B indicates autofluorescent blood vessels. *P<0.05. (D-I) msxb, msxe and aldh1a2 in situ hybridization (5 dpa). Arrowheads indicate expression of msxb, msxe and aldh1a2. (J) qPCR analysis after a 10-hour heat-shock series (62-72 hpa): msxb, msxe and aldh1a2 transcripts are increased in Tg(UAS:NICD) fins. *P<0.05. Scale bars: 10 μm in B,I.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a2 WT physical alteration: anatomical structure Adult blastema ISH
Adult regenerating fin ISH
kca3Tg; kca4Tg physical alteration: anatomical structure, heat shock Adult blastema ISH
Adult regenerating fin ISH
msx1a WT physical alteration: anatomical structure Adult blastema ISH
Adult regenerating fin ISH
kca3Tg; kca4Tg physical alteration: anatomical structure, heat shock Adult blastema ISH
Adult regenerating fin ISH
msx1b WT physical alteration: anatomical structure Adult blastema ISH
Adult regenerating fin ISH
kca3Tg; kca4Tg physical alteration: anatomical structure, heat shock Adult blastema ISH
Adult regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg; kca4Tg physical alteration: anatomical structure, heat shock Adult blastema cell population proliferation increased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development