FIGURE

Fig. 5

ID

ZDB-FIG-130510-12

Publication

M�nch et al., 2013 - Notch regulates blastema proliferation and prevents differentiation during adult zebrafish fin regeneration

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Fig. 5

Notch gain of function increases proliferation and is accompanied by an expansion of distal blastema marker expression into proximal regions. (A-C) BrdU-stained fin sections after a 10-hour heat-shock series (62-72 hpa) and quantification of the percentage of BrdU-labelled DAPI nuclei in the blastema: more cells incorporate BrdU in Tg(UAS:NICD) fins (B) than in wild-type fins (A). Asterisk in B indicates autofluorescent blood vessels. ^*P<0.05. (D-I) msxb, msxe and aldh1a2 in situ hybridization (5 dpa). Arrowheads indicate expression of msxb, msxe and aldh1a2. (J) qPCR analysis after a 10-hour heat-shock series (62-72 hpa): msxb, msxe and aldh1a2 transcripts are increased in Tg(UAS:NICD) fins. ^*P<0.05. Scale bars: 10 μm in B,I.

Expression Data

Genes:	aldh1a2 msx1a msx1b
Fish:	WT kca3Tg; kca4Tg
Conditions:	physical alteration: anatomical structure heat shock, physical alteration: anatomical structure
Anatomical Terms:	blastema regenerating fin
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kca3Tg; kca4Tg
Condition:	heat shock, physical alteration: anatomical structure
Observed In:	blastema cell population proliferation
Stage:	Adult

Phenotype Detail

Acknowledgments

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