ZFIN ID: ZDB-FIG-130510-13
Münch et al., 2013 - Notch regulates blastema proliferation and prevents differentiation during adult zebrafish fin regeneration. Development (Cambridge, England)   140(7):1402-1411 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 6

Notch signalling inhibits osteoblast differentiation and prevents bone regeneration. (A,B) Alizarin Red staining of fins at 5 dpa reveals calcified bony rays in a wild-type fin regenerate (A, arrow). Fin rays are poorly formed in Tg(UAS:NICD) fins, whereas the blastema (b) is expanded (B, arrow). (C) Length of regenerated calcified radials of Tg(UAS:NICD) fins, relative to wild-type fins. ***P<0.001. (D,E) In situ hybridization at 5 dpa. Wild-type fins express tcf7 in peripheral cells in the distal region (D, arrow), whereas transcription is expanded proximally in Tg(UAS:NICD) fins (E, arrow). (F,G) Immunostaining for Osx and Myc-NICD. In wild-type fins, Osx+ cells (green) are present in the proximal fin region but scarce in the distal region (F, bracket). Osx+ cells are less numerous in regenerating Tg(UAS:NICD) fins and more proximally limited. The region of high Myc-NICD expression does not contain Osx+ cells (G, bracket). (H) Relative length of the proximal region of the fin regenerate, which contains Osx+ cells, and the distal region (brackets, F,G), which does not (Osx-). The distal, Osx- region is larger in Tg(UAS:NICD) fins than in wild-type fins. *P<0.05. Scale bars: 200 μm in B; 10 μm in E,G. Broken lines indicate the amputation plane.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tcf7 WT physical alteration: anatomical structure Adult regenerating fin ISH
kca3Tg; kca4Tg physical alteration: anatomical structure, heat shock Adult regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg; kca4Tg physical alteration: anatomical structure, heat shock Adult blastema distended, abnormal
Adult regenerating fin caudal fin lepidotrichium malformed, abnormal
Adult regenerating fin ossification decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development