ZFIN ID: ZDB-FIG-130510-13
Münch et al., 2013 - Notch regulates blastema proliferation and prevents differentiation during adult zebrafish fin regeneration. Development (Cambridge, England)   140(7):1402-1411 Full text @ Development
Anatomical Term:
Stage: Adult
Observed In:
Stage: Adult

Fig. 6

Notch signalling inhibits osteoblast differentiation and prevents bone regeneration. (A,B) Alizarin Red staining of fins at 5 dpa reveals calcified bony rays in a wild-type fin regenerate (A, arrow). Fin rays are poorly formed in Tg(UAS:NICD) fins, whereas the blastema (b) is expanded (B, arrow). (C) Length of regenerated calcified radials of Tg(UAS:NICD) fins, relative to wild-type fins. ***P<0.001. (D,E) In situ hybridization at 5 dpa. Wild-type fins express tcf7 in peripheral cells in the distal region (D, arrow), whereas transcription is expanded proximally in Tg(UAS:NICD) fins (E, arrow). (F,G) Immunostaining for Osx and Myc-NICD. In wild-type fins, Osx+ cells (green) are present in the proximal fin region but scarce in the distal region (F, bracket). Osx+ cells are less numerous in regenerating Tg(UAS:NICD) fins and more proximally limited. The region of high Myc-NICD expression does not contain Osx+ cells (G, bracket). (H) Relative length of the proximal region of the fin regenerate, which contains Osx+ cells, and the distal region (brackets, F,G), which does not (Osx-). The distal, Osx- region is larger in Tg(UAS:NICD) fins than in wild-type fins. *P<0.05. Scale bars: 200 μm in B; 10 μm in E,G. Broken lines indicate the amputation plane.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tcf7 WT physical alteration: anatomical structure Adult regenerating fin ISH
kca3Tg; kca4Tg physical alteration: anatomical structure, heat shock Adult regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg; kca4Tg physical alteration: anatomical structure, heat shock Adult blastema distended, abnormal
Adult regenerating fin caudal fin lepidotrichium malformed, abnormal
Adult regenerating fin ossification decreased process quality, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development