ZFIN Image: Münch et al., 2013, Fig. 5

Image

Figure Caption

Fig. 5

Notch gain of function increases proliferation and is accompanied by an expansion of distal blastema marker expression into proximal regions. (A-C) BrdU-stained fin sections after a 10-hour heat-shock series (62-72 hpa) and quantification of the percentage of BrdU-labelled DAPI nuclei in the blastema: more cells incorporate BrdU in Tg(UAS:NICD) fins (B) than in wild-type fins (A). Asterisk in B indicates autofluorescent blood vessels. ^*P<0.05. (D-I) msxb, msxe and aldh1a2 in situ hybridization (5 dpa). Arrowheads indicate expression of msxb, msxe and aldh1a2. (J) qPCR analysis after a 10-hour heat-shock series (62-72 hpa): msxb, msxe and aldh1a2 transcripts are increased in Tg(UAS:NICD) fins. ^*P<0.05. Scale bars: 10 μm in B,I.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development