ZFIN ID: ZDB-FIG-130326-42
Djiotsa et al., 2012 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression. BMC Developmental Biology   12(1):37 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-15

Fig. 2 Identification of pax4 and arx expressing cells by double fluorescent in situ hybridization. All images are confocal optical sections of the dorsal pancreatic bud with anterior part to the left. The probes and the developmental stages are respectively indicated at the top and the bottom in each image. All views are ventral, except images P, Q, R and S which are lateral views. arx transcripts are detected in almost all glucagon+ (A, B), in some ghrelin+ (C, D) cells and in many isl1+ cells (H); absence of co-staining between arx and insulin (E, F) and somatostatin (G). pax4 transcripts are not detected in insulin+ (I - K) and most glucagon+ cells (L). pax4 is expressed in many somatostatin (M) and a few ghrelin (N) expressing cells. Partial co-staining of pax4 probe with a cocktail of insulin, glucagon, somatostatin and ghrelin probes (horm)(O), with isl1 (P) and sox4b (Q) probes. Absence of co-staining between pax4 and nkx6.1 (R). S: Schematic representation of the multi-layer organization of the pancreatic dorsal bud at 24 hpf including pax4 expression. Scale bar = 20 μm. astĂ©risks (*) indicate double positive cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arxa WT standard conditions Prim-5 Not Detected D cell ISH
Prim-5 ghrelin secreting cell ISH
Prim-5 glucagon secreting cell ISH
Prim-5 insulin secreting cell ISH
Prim-5 pancreatic A cell ISH
Prim-5 pancreatic B cell ISH
Prim-5 pancreatic bud ISH
Prim-5 pancreatic epsilon cell ISH
Prim-5 Not Detected somatostatin secreting cell ISH
Prim-15 Not Detected D cell ISH
Prim-15 ghrelin secreting cell ISH
Prim-15 glucagon secreting cell ISH
Prim-15 insulin secreting cell ISH
Prim-15 pancreatic A cell ISH
Prim-15 pancreatic B cell ISH
Prim-15 pancreatic bud ISH
Prim-15 pancreatic epsilon cell ISH
Prim-15 Not Detected somatostatin secreting cell ISH
gcga WT standard conditions Prim-5 glucagon secreting cell ISH
Prim-5 pancreatic A cell ISH
Prim-15 glucagon secreting cell ISH
Prim-15 pancreatic A cell ISH
ghrl WT standard conditions Prim-5 ghrelin secreting cell ISH
Prim-5 pancreatic epsilon cell ISH
Prim-15 ghrelin secreting cell ISH
Prim-15 pancreatic epsilon cell ISH
ins WT standard conditions 14-19 somites insulin secreting cell ISH
14-19 somites pancreatic B cell ISH
20-25 somites insulin secreting cell ISH
20-25 somites pancreatic B cell ISH
Prim-5 insulin secreting cell ISH
Prim-5 pancreatic B cell ISH
Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
isl1a WT standard conditions Prim-5 insulin secreting cell ISH
Prim-5 pancreatic B cell ISH
Prim-5 pancreatic bud ISH
Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
Prim-15 pancreatic bud ISH
nkx6.1 WT standard conditions Prim-5 posterior pancreatic bud ISH
pax4 WT standard conditions 14-19 somites Not Detected insulin secreting cell ISH
14-19 somites Not Detected pancreatic B cell ISH
20-25 somites Not Detected insulin secreting cell ISH
20-25 somites Not Detected pancreatic B cell ISH
Prim-5 D cell ISH
Prim-5 ghrelin secreting cell ISH
Prim-5 glucagon secreting cell ISH
Prim-5 Not Detected insulin secreting cell ISH
Prim-5 pancreatic A cell ISH
Prim-5 Not Detected pancreatic B cell ISH
Prim-5 pancreatic epsilon cell ISH
Prim-5 somatostatin secreting cell ISH
sox4b WT standard conditions Prim-5 posterior pancreatic bud ISH
sst2 WT standard conditions Prim-15 D cell ISH
Prim-15 somatostatin secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.